Liu K P, Su Y W, Zhang J W, Wang Z, Ma Y Y, Liu Y M, Xiao Y M
Guangzhou Twelfth People's Hospital Affiliated to Guangzhou Medical University, Guangzhou 510620, China.
Guangzhou Occupational Disease Prevention and Treatment Hospital, Guangzhou 510620, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2021 Jan 20;39(1):44-47. doi: 10.3760/cma.j.cn121094-20200115-00030.
To study the changes of proliferation and oxidation indexes of Cochlear hair cell line (HEI-OC1 cells) exposed to ethylbenzene. From July to December 2019, 11 groups with ethylbenzene concentrations of 0, 30, 60, 90, 300, 600, 900 μmol/L and 3, 6, 9, 10 mmol/L, were used to determine the proliferation activity of HEI-OC1 cells exposed to ethylbenzene for 24 hours, and the cells were treated with 0, 1, 2, 4, 8, 16, 32, 64 mmol/L ethylbenzene for 24 hours, then the 50% inhibitory concentration of ethylbenzene was calculated. After HEI-OC1 cells were exposed to 0, 6, 9 and 12 mmol/L ethylbenzene for 24 hours, the malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured. Compared with 0 mmol/L concentration group, the survival rate of HEI-OC1 cells at 6, 9, 12 mmol/L concentration was significantly decreased (<0.01) . The 50% inhibitory concentration of ethylbenzene on HEI-OC1 cells was 12.86 mmol/L ((2)=99.05) . There were significant differences in SOD and GSH-Px activity in HEI-OC1 cells treated with ethylbenzene at different concentrations (0, 6, 9, 12 mmol/L) for 24 hours (=65.11, 6.48, 22.85, <0.05) . Compared with 0 mmol/L concentration group, the MDA content of HEI-OC1 cells was significantly increased in 9 and 12 mmol/L concentration groups, the SOD activity was significantly decreased in 12 mmol/L concentration group, and the GSH-Px activity was significantly decreased in 6 and 12 mmol/L concentration groups. Ethylbenzene can inhibit the proliferation of HEI-OC1 cells and cause oxidative damage.
研究乙苯暴露下耳蜗毛细胞系（HEI-OC1细胞）增殖及氧化指标的变化。2019年7月至12月，设置乙苯浓度为0、30、60、90、300、600、900 μmol/L以及3、6、9、10 mmol/L的11个组，测定乙苯暴露24小时的HEI-OC1细胞增殖活性，并用0、1、2、4、8、16、32、64 mmol/L乙苯处理细胞24小时，计算乙苯的50%抑制浓度。将HEI-OC1细胞用0、6、9和12 mmol/L乙苯暴露24小时后，测定丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。与0 mmol/L浓度组相比，6、9、12 mmol/L浓度时HEI-OC1细胞存活率显著降低（<0.01）。乙苯对HEI-OC1细胞的50%抑制浓度为12.86 mmol/L（(2)=99.05）。不同浓度（0、6、9、12 mmol/L）乙苯处理24小时的HEI-OC1细胞中SOD和GSH-Px活性存在显著差异（=65.11、6.48、22.85，<0.05）。与0 mmol/L浓度组相比，9和12 mmol/L浓度组HEI-OC1细胞的MDA含量显著增加，12 mmol/L浓度组SOD活性显著降低，6和12 mmol/L浓度组GSH-Px活性显著降低。乙苯可抑制HEI-OC1细胞增殖并造成氧化损伤。
