Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Center for Advanced Light Microscopy, Ghent University, 9000 Ghent, Belgium.
Int J Mol Sci. 2021 Feb 1;22(3):1434. doi: 10.3390/ijms22031434.
Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.
核糖体失活蛋白（RIPs）是一类细胞毒性酶，可通过脱嘌呤 rRNA 抑制蛋白质翻译。大多数植物 RIPs 合成时有一个信号序列，将蛋白质隔离在远离宿主核糖体的细胞区室中。然而，一些水稻 RIPs 缺乏这些信号肽，表明它们存在于细胞质中，靠近植物核糖体。本文旨在阐明两种来自水稻的核质 RIP 的生理功能，特别是被称为 OsRIP1 的 1 型 RIP 和一种假定的 3 型 RIP，称为 nuRIP。构建了过量表达这些 RIP 的转基因水稻系，并在温室条件下研究了由于这种过表达而导致的发育效应。此外，还研究了转基因幼苗对干旱、盐、脱落酸和茉莉酸甲酯处理的反应性能。结果表明，这两种 RIP 都可以影响茉莉酸甲酯介导的应激反应。
