高级显微镜在 HIV 及其与宿主相互作用研究中的应用。
Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host.
机构信息
Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.
出版信息
Viruses. 2021 Feb 1;13(2):223. doi: 10.3390/v13020223.
Abstract
This review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques. The advances in technology which now enables these light microscopy measurements are discussed with special emphasis on live imaging approaches including Total Internal Reflection Fluorescence (TIRF), high-resolution light microscopy techniques including PALM and STORM and single molecule measurements, including Fluorescence Resonance Energy Transfer (FRET). The review concludes with a discussion on what new insights and understanding can be expected from these measurements.
摘要
本综述强调了借助先进的光学显微镜技术在人类免疫缺陷病毒 (HIV) 组装、释放和成熟方面的重要观察结果。本文讨论了使这些光学显微镜测量成为可能的技术进步，特别强调了包括全内反射荧光 (TIRF) 在内的活细胞成像方法、PALM 和 STORM 等高分辨率光学显微镜技术以及包括荧光共振能量转移 (FRET) 在内的单分子测量。本文最后讨论了可以从这些测量中获得哪些新的见解和认识。
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