Department of Animal Science and Aquaculture, Dalhousie University, Truro, NS, Canada.
Department of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, Canada.
Sci Rep. 2021 Feb 3;11(1):2944. doi: 10.1038/s41598-021-82522-8.
Aleutian disease (AD) is the most significant health issue for farmed American mink. The objective of this study was to identify the genomic regions subjected to selection for response to infection with Aleutian mink disease virus (AMDV) in American mink using genotyping by sequencing (GBS) data. A total of 225 black mink were inoculated with AMDV and genotyped using a GBS assay based on the sequencing of ApeKI-digested libraries. Five AD-characterized phenotypes were used to assign animals to pairwise groups. Signatures of selection were detected using integrated measurement of fixation index (F) and nucleotide diversity (θπ), that were validated by haplotype-based (hap-FLK) test. The total of 99 putatively selected regions harbouring 63 genes were detected in different groups. The gene ontology revealed numerous genes related to immune response (e.g. TRAF3IP2, WDR7, SWAP70, CBFB, and GPR65), liver development (e.g. SULF2, SRSF5) and reproduction process (e.g. FBXO5, CatSperβ, CATSPER4, and IGF2R). The hapFLK test supported two strongly selected regions that contained five candidate genes related to immune response, virus-host interaction, reproduction and liver regeneration. This study provided the first map of putative selection signals of response to AMDV infection in American mink, bringing new insights into genomic regions controlling the AD phenotypes.
阿留申病（AD）是养殖北美水貂最重要的健康问题。本研究的目的是使用基于 ApeKI 酶切文库测序的 GBS 数据，鉴定对感染阿留申病病毒（AMDV）有反应的基因组区域。总共 225 只黑色水貂接种了 AMDV，并使用基于 GBS 的 Assay 进行了基因分型。使用 AD 特征表型将动物分配到两两分组中。通过固定指数（F）和核苷酸多样性（θπ）的综合测量来检测选择的特征，并通过基于单倍型的（hap-FLK）测试进行验证。在不同的分组中检测到了总共 99 个可能被选择的含有 63 个基因的区域。基因本体论揭示了许多与免疫反应（例如 TRAF3IP2、WDR7、SWAP70、CBFB 和 GPR65）、肝脏发育（例如 SULF2、SRSF5）和生殖过程（例如 FBXO5、CatSperβ、CATSPER4 和 IGF2R）相关的基因。hap-FLK 测试支持两个强烈选择的区域，其中包含五个与免疫反应、病毒-宿主相互作用、生殖和肝脏再生有关的候选基因。本研究提供了北美水貂对 AMDV 感染反应的潜在选择信号的第一张图谱，为控制 AD 表型的基因组区域提供了新的见解。
