Department of Animal Science and Aquaculture, Dalhousie University, Bible Hill, NS, B2N5E3, Canada.
Department of Animal Science, University of Zanjan, Zanjan, 4537138791, Zanjan, Iran.
Sci Rep. 2024 Jan 10;14(1):1035. doi: 10.1038/s41598-023-51039-7.
Aleutian disease (AD) is a multi-systemic infectious disease in American mink (Neogale vison) caused by Aleutian mink disease virus (AMDV). This study aimed to identify candidate regions and genes underlying selection for response against AMDV using whole-genome sequence (WGS) data. Three case-control selection signatures studies were conducted between animals (N = 85) producing high versus low antibody levels against AMDV, grouped by counter immunoelectrophoresis (CIEP) test and two enzyme-linked immunosorbent assays (ELISA). Within each study, selection signals were detected using fixation index (FST) and nucleotide diversity (θπ ratios), and validated by cross-population extended haplotype homozygosity (XP-EHH) test. Within- and between-studies overlapping results were then evaluated. Within-studies overlapping results indicated novel candidate genes related to immune and cellular responses (e.g., TAP2, RAB32), respiratory system function (e.g., SPEF2, R3HCC1L), and reproduction system function (e.g., HSF2, CFAP206) in other species. Between-studies overlapping results identified three large segments under strong selection pressure, including two on chromosome 1 (chr1:88,770-98,281 kb and chr1:114,133-120,473) and one on chromosome 6 (chr6:37,953-44,279 kb). Within regions with strong signals, we found novel candidate genes involved in immune and cellular responses (e.g., homologous MHC class II genes, ITPR3, VPS52) in other species. Our study brings new insights into candidate regions and genes controlling AD response.
阿留申病（AD）是一种由阿留申病病毒（AMDV）引起的美国水貂（Neogale vison）多系统传染性疾病。本研究旨在使用全基因组序列（WGS）数据鉴定对 AMDV 产生反应的选择背后的候选区域和基因。在 3 项病例对照选择标志研究中，根据免疫电泳（CIEP）试验和 2 种酶联免疫吸附试验（ELISA），将产生针对 AMDV 的高抗体水平与低抗体水平的动物（N=85）分为两组，比较两组间的选择信号。在每项研究中，通过固定指数（FST）和核苷酸多样性（θπ 比值）检测选择信号，并通过交叉群体扩展单倍型同质性（XP-EHH）试验进行验证。然后评估了研究内和研究间重叠结果。研究内重叠结果表明，在其他物种中，与免疫和细胞反应（如 TAP2、RAB32）、呼吸系统功能（如 SPEF2、R3HCC1L）和生殖系统功能（如 HSF2、CFAP206）相关的新型候选基因。研究间重叠结果确定了三个受到强烈选择压力的大区域，包括两个在 1 号染色体上（chr1：88770-98281 kb 和 chr1：114133-120473）和一个在 6 号染色体上（chr6：37953-44279 kb）。在具有强烈信号的区域内，我们发现了其他物种中与免疫和细胞反应相关的新型候选基因（如同源 MHC 类 II 基因、ITPR3、VPS52）。本研究为控制 AD 反应的候选区域和基因提供了新的见解。
