Arias David, Rodríguez Johny, López Betty, Méndez Paula
Grupo de Investigación en Etnofarmacología, Productos Naturales y Alimentos, Escuela de Ciencias Básicas Tecnología e Ingeniería, Universidad Nacional Abierta y a Distancia, Calle 14 Sur # 14 - 23, Bogotá, Colombia.
Grupo de Investigación Ciencia de los Materiales, Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Calle 70 N° 52-21, Medellín, Colombia.
Heliyon. 2021 Jan 24;7(1):e06059. doi: 10.1016/j.heliyon.2021.e06059. eCollection 2021 Jan.
Colombia is one of the major producers and exporters of banana . Its consumption is high then its agro-industrial wastes are important source to extract pectin. In the present study, inorganic acids (nitric acid and hydrochloride acid) and organic acids (citric acid and acetic acid) were evaluated to extract pectin from banana peels at pH 2 and 3, through acid hydrolysis during 1h at the temperature of 85 °C. The extraction yield, degree of esterification (DE) and intrinsic viscosity of the pectin were characterized. Nitric acid (NA) allowed to obtain higher extraction yield (54 ± 10%) and citric acid (CA) allowed to obtain higher DE (76.7 ± 2%). Additionally, the effect of polymer concentration and degree esterification, low degree of esterification (LDE, 33.1%) and high degree of esterification (HDE, 76.7%) were evaluated in the pectin nanoparticles formation. The pectin with HDE formed stable nanoparticles with a particle size of 255 ± 11 nm and the zeta potential value of -45 ± 3 mV, independently of the polymer concentration. Morphology analysis showed aggregated of the pectin nanoparticles.
哥伦比亚是香蕉的主要生产国和出口国之一。其消费量很高，因此其农业工业废弃物是提取果胶的重要来源。在本研究中，评估了无机酸（硝酸和盐酸）和有机酸（柠檬酸和乙酸）在pH值为2和3的条件下，于85℃温度下通过酸水解1小时从香蕉皮中提取果胶的效果。对果胶的提取率、酯化度（DE）和特性粘度进行了表征。硝酸（NA）可获得较高的提取率（54±10%），柠檬酸（CA）可获得较高的DE（76.7±2%）。此外，还评估了聚合物浓度和酯化度、低酯化度（LDE，33.1%）和高酯化度（HDE，76.7%）对果胶纳米颗粒形成的影响。具有HDE的果胶形成了稳定的纳米颗粒，粒径为255±11nm，ζ电位值为-45±3mV，与聚合物浓度无关。形态分析显示果胶纳米颗粒发生了聚集。
