Cheon Yong-Pil, Ko CheMyong, Lee Ki-Ho
Division of Development and Physiology, Department of Biotechnology, CDPR, Institute for Basic Science, Sungshin Women University, Seoul, 02844 Korea.
Dept. of Comparative Biosciences, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA.
Dev Reprod. 2020 Dec;24(4):287-296. doi: 10.12717/DR.2020.24.4.287. Epub 2020 Dec 31.
The absence of functional estrogen receptor α (Esr1) results in an overgrowth of the epididymal fat, as observed in estrogen receptor α knockout (ERαKO) mouse. The present research was aimed to evaluate expression of various molecules associated with adipocyte differentiation and maturation in the epididymal fat of ERαKO mouse at several postnatal ages by using quantitative real-time polymerase chain reaction. The highest transcript levels of all molecules were detected at 12 months of postnatal age, except leptin which the mRNA level was increased at 5 months of age and was unchanged until 12 months of age. The expression levels of CCAAT enhancer binding protein (Cebp) alpha, androgen receptor, and lipoprotein lipase were decreased at 5 months of age but increased at about 8 months of age. The mRNA levels of Cebp gamma and sterol regulatory element binding transcription factor 1 remained steady until 8 months of age. Continuous increases of transcript levels during postnatal period were found in Cebp beta, estrogen receptor (ER) beta, fatty acid binding protein 4, and delta like non-canonical Notch ligand 1. The increases of peroxisome proliferator-activated receptor gamma and adiponectin mRNA levels were detected as early as 8 months of age. The levels of fatty acid synthase and resistin transcript at 5 and 8 months of age were lower than that at 2 months of age. These findings show the aberrant expression patterns of genes related to adipocyte differentiation and maturation in the postnatal epididymal fat pad by the disruption of ER alpha function.
如在雌激素受体α基因敲除（ERαKO）小鼠中所观察到的，功能性雌激素受体α（Esr1）的缺失会导致附睾脂肪过度生长。本研究旨在通过定量实时聚合酶链反应评估不同出生后年龄的ERαKO小鼠附睾脂肪中与脂肪细胞分化和成熟相关的各种分子的表达。除瘦素外，所有分子的最高转录水平在出生后12个月时检测到，瘦素的mRNA水平在5个月龄时升高，直到12个月龄时保持不变。CCAAT增强子结合蛋白（Cebp）α、雄激素受体和脂蛋白脂肪酶的表达水平在5个月龄时降低，但在约8个月龄时升高。Cebpγ和固醇调节元件结合转录因子1的mRNA水平在8个月龄之前保持稳定。在出生后时期，Cebpβ、雌激素受体（ER）β、脂肪酸结合蛋白4和类Notch配体1的转录水平持续增加。过氧化物酶体增殖物激活受体γ和脂联素mRNA水平早在8个月龄时就被检测到升高。5个月龄和8个月龄时脂肪酸合酶和抵抗素转录水平低于2个月龄时。这些发现表明，由于ERα功能的破坏，出生后附睾脂肪垫中与脂肪细胞分化和成熟相关的基因存在异常表达模式。
