Dieudonne M N, Pecquery R, Leneveu M C, Jaubert A M, Giudicelli Y
Department of Biochemistry, INSERM CJF (94-02), Faculty of Medicine Paris-Ouest, Université Paris V, C.H.I., 78303, Poissy Cedex, France.
Endocrine. 1995 Jul;3(7):537-41. doi: 10.1007/BF02738830.
Different studies suggest that sex hormones affect adipose tissue metabolism and deposition. To investigate the possibility that androgens may play a role in adipose tissue development, we have studied androgen receptors (AR) in rat adipose precursor cells from two different anatomical fat deposits, one deep intraabdominal (epididymal) and one subcutaneous (inguinal) during the proliferation and differentiation processes. AR were quantified by [(3)H]R1881 specific binding in whole cells and the nuclear fraction and were localized by immunocytofluorimetry in both the cytosol and the nucleus. During the proliferative phase, total AR level decreased from D3 to D6. At confluence (D5), AR were higher in epididymal (64±4 fmol/mg protein) than in subcutaneous (33±3 fmoles/mg proteins) preadipocytes and were up-regulated by testosterone but not by 5α-dihydrotestosterone or by 17β-estradiol. At differentiation (D10-11), nuclear AR decreased by 50% in both precursor fat cell populations when compared to the confluent state (D5) and AR were no more up-regulated but rather down-regulated by testosterone. Because AR are present in preadipocytes and are differently regulated by testosterone depending on the stage of proliferation and differentiation, this study suggests that testosterone may play a role in the control of the adipogenic process.
不同的研究表明，性激素会影响脂肪组织的代谢和沉积。为了探究雄激素可能在脂肪组织发育中发挥作用的可能性，我们研究了来自大鼠两个不同解剖学脂肪沉积部位（一个是腹内深部（附睾）脂肪，另一个是皮下（腹股沟）脂肪）的脂肪前体细胞在增殖和分化过程中的雄激素受体（AR）。通过全细胞和细胞核组分中的[(3)H]R1881特异性结合对AR进行定量，并通过免疫细胞荧光法在细胞质和细胞核中定位。在增殖期，总AR水平从第3天到第6天下降。在汇合期（第5天），附睾前脂肪细胞（64±4 fmol/mg蛋白质）中的AR高于皮下前脂肪细胞（33±3 fmol/mg蛋白质），并且睾酮可上调AR，但5α-二氢睾酮或17β-雌二醇则不能。在分化期（第10 - 11天），与汇合状态（第5天）相比，两个前体脂肪细胞群体中的细胞核AR均下降了50%，并且AR不再被睾酮上调，反而被其下调。由于AR存在于前脂肪细胞中，并且根据增殖和分化阶段受到睾酮的不同调节，本研究表明睾酮可能在脂肪生成过程的控制中发挥作用。
