Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
J Econ Entomol. 2021 Apr 13;114(2):947-958. doi: 10.1093/jee/toab004.
We used transcriptome analysis to research ovary development in Bactrocera dorsalis (Hendel). The ovary transcriptome of B. dorsalis yielded 66,463,710 clean reads that were assembled into 23,822 unigenes. After aligning to the Nr database in NCBI, 15,473 (64.95%) of the unigenes were matched to identified proteins. As determined by BLAST search, 11,043 (46.36%), 6,102 (25.61%), and 12,603 (52.90%) unigenes were each allocated to clusters via gene ontology, orthologous groups, and SwissProt, respectively. The Kyoto encyclopedia database of genes and genomes (KEGG) was further used to annotate these sequences, and 11,068 unigenes were mapped to 255 known pathways. Afterward, the genes that were possibly involved in oogenesis and ovary development were obtained from the transcriptome data and analyzed. Interestingly, seven ovary-specific genes were identified, including a Nanos gene that is involved in maintaining the primordial germ cells in many insects. Therefore, we further focused on the function of the BdNanos gene, and the gene was injected into B. dorsalis. As expected, the knocking down of Nanos gene expression led to significant inhibition of ovary development, suggesting an important role of this gene in the reproductive process of B. dorsalis. In summary, the present study provides an important reference for identifying the molecular mechanisms of oogenesis and ovary development in B. dorsalis. The BdNanos gene is crucial for ovary development in B. dorsalis and is therefore a potential new pest control target.
我们使用转录组分析来研究东方果实蝇（Hendel）的卵巢发育。东方果实蝇的卵巢转录组产生了 66463710 条清洁读数，这些读数被组装成 23822 条非编码 RNA。在将这些非编码 RNA 与 NCBI 中的 Nr 数据库对齐后，15473（64.95%）条非编码 RNA 与已鉴定的蛋白质相匹配。通过 BLAST 搜索确定，11043（46.36%）、6102（25.61%）和 12603（52.90%）条非编码 RNA 分别通过基因本体、直系同源群和 SwissProt 分配到聚类中。京都基因与基因组百科全书（KEGG）进一步用于注释这些序列，11068 条非编码 RNA 映射到 255 个已知途径。之后，从转录组数据中获得了可能参与卵子发生和卵巢发育的基因，并进行了分析。有趣的是，鉴定出了 7 个卵巢特异性基因，包括一个参与维持许多昆虫原始生殖细胞的 Nanos 基因。因此，我们进一步关注 BdNanos 基因的功能，并将该基因注入东方果实蝇。不出所料，Nanos 基因表达的敲低导致卵巢发育显著抑制，表明该基因在东方果实蝇生殖过程中起着重要作用。总之，本研究为鉴定东方果实蝇卵子发生和卵巢发育的分子机制提供了重要参考。BdNanos 基因对东方果实蝇的卵巢发育至关重要，因此是一个潜在的新害虫控制靶标。
