Department of Otorhinolaryngology‑Head and Neck Surgery, Wuhan Puren Hospital, Wuhan, Hubei 430081, P.R. China.
Department of Otorhinolaryngology‑Head and Neck Surgery, Wuhan University Renmin Hospital, Wuhan, Hubei 430060, P.R. China.
Mol Med Rep. 2021 Apr;23(4). doi: 10.3892/mmr.2021.11882. Epub 2021 Feb 4.
Several studies on papillary thyroid cancer (PTC) have been performed. However, the effects of endothelin 3 (EDN3) and microRNA (miR)‑27a‑3p on PTC cells has yet to be investigated, to the best of the authors' knowledge. The present study aimed to explore the biological functions of EDN3 and miR‑27a‑3p in PTC cells. Bioinformatics analysis was conducted to identify possible key genes and miRs involved in PTC progression. Western blot analysis and reverse transcription‑quantitative (RT‑q) PCR were employed to confirm the key genes or miRs expressed in PTC cells. Cytological methods were used to detect cell viability, proliferation, apoptosis and migration and luciferase reporter assay was performed to confirm the relationship between END3 and miR‑27a‑3p. After analyzing the results of gene microarray analyses and RT‑qPCR, EDN3 with low expression was identified as the key gene associated with PTC progression. It was also found that EDN3 overexpression in PTC cells impaired cell viability, proliferation and migration but promoted cell apoptosis. In addition, the findings revealed that miR‑27a‑3p could relieve the inhibitory influence of EDN3 on PTC cells by binding to EDN3 mRNA 3' untranslated region (UTR), thereby suppressing EDN3 expression. Overall, the results of the present study demonstrated that by binding to EDN3 mRNA 3'UTR, miR‑27a‑3p could attenuate the inhibitory function of EDN3 in the tumorigenesis of PTC cells.
已有多项关于甲状腺乳头状癌（PTC）的研究。然而，据作者所知，内皮素 3（EDN3）和 microRNA（miR）-27a-3p 对 PTC 细胞的影响尚未得到研究。本研究旨在探讨 EDN3 和 miR-27a-3p 在 PTC 细胞中的生物学功能。通过生物信息学分析鉴定可能参与 PTC 进展的关键基因和 miR。采用 Western blot 分析和逆转录-定量（RT-q）PCR 验证 PTC 细胞中表达的关键基因或 miR。采用细胞学方法检测细胞活力、增殖、凋亡和迁移，采用荧光素酶报告基因检测证实 END3 和 miR-27a-3p 之间的关系。在分析基因微阵列分析和 RT-qPCR 的结果后，发现低表达的 EDN3 是与 PTC 进展相关的关键基因。研究还发现，EDN3 在 PTC 细胞中的过表达可损害细胞活力、增殖和迁移，但促进细胞凋亡。此外，研究结果表明，miR-27a-3p 可通过结合 EDN3 mRNA 3'非翻译区（UTR）来缓解 EDN3 对 PTC 细胞的抑制作用，从而抑制 EDN3 的表达。综上所述，本研究结果表明，miR-27a-3p 通过结合 EDN3 mRNA 3'UTR 可减弱 EDN3 在 PTC 细胞肿瘤发生中的抑制作用。
