Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam 50612, Republic of Korea.
College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Mol Med Rep. 2021 Apr;23(4). doi: 10.3892/mmr.2021.11887. Epub 2021 Feb 4.
Alisol B 23‑acetate (AB23A) is a natural triterpenoid isolated from , which exhibits a number of pharmacological activities. In the present study, AB23A‑induced anticancer efficacy was examined in AGS gastric cancer cells. Cell viability assay, cell cycle analysis, caspase activity assay, western blotting and reactive oxygen species (ROS) assay were used to investigate the anticancer effects of AB23A on AGS cells. AB23A reduced the viability of AGS cells, increased the sub‑G1 cell fraction and depolarized the mitochondrial membrane. Notably, AB23A‑induced cell death was associated with downregulation of the B‑cell lymphoma 2 and survivin proteins, and upregulation of the Bax protein. In addition, AB23A increased caspase‑3 and ‑9 activities, and regulated the activation of mitogen‑activated protein kinases (MAPK). Moreover, AB23A increased the production of reactive oxygen species. These results suggested that AB23A may induce apoptosis through cell cycle arrest and the mitochondrial pathway, accompanied by the caspase and MAPK signaling cascades. In conclusion, AB23A may have potential as a novel anticancer drug for the treatment of gastric cancer.
阿立醇 B23- 醋酸酯（AB23A）是从 中分离得到的一种天然三萜类化合物，具有多种药理活性。本研究考察了 AB23A 对 AGS 胃癌细胞的抗癌作用。细胞活力测定、细胞周期分析、半胱氨酸天冬氨酸蛋白酶（caspase）活性测定、Western blot 分析和活性氧（ROS）测定用于研究 AB23A 对 AGS 细胞的抗癌作用。AB23A 降低 AGS 细胞活力，增加亚 G1 细胞部分，并使线粒体膜去极化。值得注意的是，AB23A 诱导的细胞死亡与 B 细胞淋巴瘤 2 和生存素蛋白下调以及 Bax 蛋白上调有关。此外，AB23A 增加了 caspase-3 和 caspase-9 的活性，并调节丝裂原激活蛋白激酶（MAPK）的激活。此外，AB23A 增加了活性氧的产生。这些结果表明，AB23A 可能通过细胞周期阻滞和线粒体途径诱导细胞凋亡，同时伴随着半胱氨酸天冬氨酸蛋白酶和 MAPK 信号级联。综上所述，AB23A 可能具有作为治疗胃癌的新型抗癌药物的潜力。
