Katagiri Hiroki, El Tawil Yacine, Lang Niklaus P, Imber Jean-Claude, Sculean Anton, Fujioka-Kobayashi Masako, Saulacic Nikola
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, Faculty of Medicine, University of Berne, CH-3010 Berne, Switzerland.
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan.
Biomedicines. 2021 Feb 2;9(2):143. doi: 10.3390/biomedicines9020143.
The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM.
本研究的目的是评估在基于胶原蛋白的基质（CM）中添加羟基磷灰石（HA）以及放置膜对颅骨缺损骨形成的影响。将16只新西兰白兔颅骨的临界尺寸缺损随机用CM或矿化胶原蛋白基基质（mCM）处理。一半的部位覆盖有胶原膜。愈合12周后对动物实施安乐死。通过显微CT和组织学对样本进行研究。在缺损周边的所有样本中均观察到新形成的板层骨。然而，在中央区域，由编织骨和板层骨组成的新骨嵌入软组织中。与CM样本相比，用mCM处理的样本在缺损中央区域显示出更多的残余生物材料，并诱导出更多的小骨岛。尽管如此，在12周时任何样本均未观察到缺损完全闭合。放置膜导致骨密度和骨高度降低。仅在缺损中央区域有膜覆盖和无膜覆盖的CM组之间，就骨高度而言，组间存在显著差异。在这个特定的缺损中，CM的矿化和膜的放置均未改善成骨能力。然而，矿化在未放置膜时影响骨密度，在放置膜时影响其下方的骨体积。CM可作为骨再生程序中的支架，无需膜覆盖。需要进一步的临床前研究来优化mCM的潜力。
