Sadat-Marashi Zahra, Fujioka-Kobayashi Masako, Katagiri Hiroki, Lang Niklaus P, Saulacic Nikola
Department of Cranio-Maxillofacial Surgery, Faculty of Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan.
Clin Oral Implants Res. 2024 Dec;35(12):1585-1596. doi: 10.1111/clr.14345. Epub 2024 Aug 21.
Collagen membranes are extensively used for guided bone regeneration procedures, primarily for horizontal bone augmentation. More recently, it has been demonstrated that collagen membranes promote bone regeneration. Present study aimed at assessing if structural modifications of collagen membranes may enhance their osteoconductive capacity.
Twenty-four adult Wistar rats were used. Bilateral calvaria defects with a diameter of 5 mm were prepared and covered with prototypes of collagen membranes (P1 or P2). The P1 membrane (positive control) presented a lower onset temperature of protein denaturation and a higher solubility than the P2 membrane (test). The contralateral defects were left uncovered (NC). After 1 and 4 weeks, the animals were euthanized. A microcomputed tomography analysis of the harvested samples was performed within and above the bony defect. Undecalcified ground sections were subjected to light microscopy and morphometric analysis.
Bone formation was observed starting from the circumferential borders of the defects in all groups at 1-week of healing. The foci of ossification were observed at the periosteal and dura mater sites, with signs of collagen membrane mineralization. However, there was no statistically significant difference between the groups. At 4 weeks, remnants of the collagen fibers were embedded in the newly formed bone. In the P2 group, significantly more bone volume, more new bone, and marrow spaces compared to the NC group were observed. Furthermore, the P2 group showed more bone volume ectocranially then the P1 group.
Bone formation subjacent to a P2 membrane was superior than subjacent to the P1 membrane and significantly better compared to the control. Modifications of the physico-chemical properties may enhance the osteoconductive competence of collagen membranes, supporting bone formation outside the bony defects.
胶原膜广泛应用于引导骨再生手术，主要用于水平骨增量。最近，已证明胶原膜可促进骨再生。本研究旨在评估胶原膜的结构修饰是否可增强其骨传导能力。
使用24只成年Wistar大鼠。制备直径为5毫米的双侧颅骨缺损，并用胶原膜原型（P1或P2）覆盖。P1膜（阳性对照）的蛋白质变性起始温度较低，溶解度高于P2膜（测试组）。对侧缺损不覆盖（NC）。1周和4周后，对动物实施安乐死。对收获的样本在骨缺损内及上方进行显微计算机断层扫描分析。对未脱钙的磨片进行光学显微镜检查和形态计量分析。
在愈合1周时，所有组均从缺损的周边边缘开始观察到骨形成。在骨膜和硬脑膜部位观察到骨化灶，有胶原膜矿化迹象。然而，各组之间无统计学显著差异。在4周时，胶原纤维残余物嵌入新形成的骨中。与NC组相比，在P2组中观察到明显更多的骨体积、更多的新骨和骨髓腔。此外，P2组颅外的骨体积比P1组更多。
P2膜下方的骨形成优于P1膜下方，且与对照组相比明显更好。物理化学性质的修饰可能增强胶原膜的骨传导能力，支持骨缺损外的骨形成。
