Polimeno Lorenzo, Lisanti Maria Teresa, Rossini Margherita, Giacovazzo Edoardo, Polimeno Lucrezia, Debellis Lucantonio, Ballini Andrea, Topi Skender, Santacroce Luigi
Polypheno Academic Spin Off, University of Bari "A. Moro", 70124 Bari, Italy.
Department of Biosciences, Biotechnologies and Biopharmaceutics, Campus Universitario "Ernesto Quagliariello", University of Bari Aldo Moro, 70125 Bari, Italy.
Biology (Basel). 2021 Feb 2;10(2):106. doi: 10.3390/biology10020106.
( infection, in humans, causes a series of clinical manifestations affecting the gastro-intestinal tract known as Anisakiasis/Anisakidosis. Patients may also present allergic manifestations such as hives and/or angioedema and even anaphylactic shock. The aim of this study was to investigate whether aquacultured fish could be considered -free food and constitute a safe, alternative, wild-capture fish food for Gastro-Allergic Anisakiasis (GAA)-sensitized subjects.
Protein extracts from larvae in the third stage (L3) and from edible part of heavily infected horse mackerel () and aquacultured sea bream, have been tested for allergens presence by immunological analysis. Western blot analysis using, as source of specific allergens antibodies, serum samples from subjects referring allergic symptoms after raw fish ingestion, was performed. These subjects showed high levels of specific IgE anti allergens determined by clinical laboratory tests (ISAC test).
Our data demonstrate the presence of Ani s4 allergen in both infected and aquacultured fish extracts, providing a possible interpretation for the allergic manifestations reported by subjects, already sensitized to , who ate frozen or well-cooked or, even, aquacultured fish.
The present data stimulate more accurate prophylaxis suggestions for allergy and more specific controls of fishmeal used in aquaculture.
（感染）在人类中会引发一系列影响胃肠道的临床表现，称为异尖线虫病。患者还可能出现过敏表现，如荨麻疹和/或血管性水肿，甚至过敏性休克。本研究的目的是调查水产养殖的鱼类是否可被视为无（异尖线虫）食物，并为对胃肠道过敏性异尖线虫病（GAA）敏感的人群构成一种安全的、替代野生捕捞鱼类的食物。
通过免疫分析检测了来自第三期幼虫（L3）以及来自严重感染的竹荚鱼和水产养殖的海鲷可食用部分的蛋白质提取物中是否存在异尖线虫过敏原。使用食用生鱼后出现过敏症状的受试者的血清样本作为特异性异尖线虫过敏原抗体的来源进行了蛋白质印迹分析。这些受试者通过临床实验室检测（ISAC检测）显示出高水平的抗异尖线虫过敏原特异性IgE。
我们的数据表明，在受感染和水产养殖的鱼类提取物中均存在Ani s4过敏原，这为已对（异尖线虫）致敏的受试者食用冷冻或煮熟的甚至是水产养殖的鱼类后报告的过敏表现提供了一种可能的解释。
目前的数据促使人们针对异尖线虫过敏提出更准确的预防建议，并对水产养殖中使用的鱼粉进行更具体的管控。
