Gao Xiuge, Teng Pei, Peng Lin, Ji Hui, Qiu Yawei, Liu Xiaoxiao, Guo Dawei, Jiang Shanxiang
Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Foods. 2021 Feb 2;10(2):301. doi: 10.3390/foods10020301.
Maduramicin (MAD) is widely introduced into aquatic environments and results in the contamination of fish products. Worryingly, the consumption of MAD-contaminated crayfish () may induce symptoms of Haff disease. In this study, to monitor this potential contamination and to understand the residue and elimination characteristics of MAD in edible tissues of crayfish, a sensitive and efficient ultra-performance liquid chromatography-tandem mass spectrometry method was developed, validated, and applied. After extraction with acetonitrile and purification by solid-phase extraction column, multiple-reaction monitoring mass spectrometry with positive ionization mode was used to determine MAD's residues. The limits of detection and of quantification were 6 μg·kg and 20 μg·kg, respectively. The fortified recoveries ranged from 74.2% to 110.4%, with relative standard deviation of 1.2% to 10.1%. Furthermore, MAD was completely eliminated after 3 and 5 days from abdominal muscle and hepatopancreas tissues of crayfish, respectively. The maximum residue limits (MRLs) of MAD respectively was 200 μg·kg in muscle and 600 μg·kg in the hepatopancreas, and its withdrawal time in both edible tissues was 25.8 °C·d. Collectively, the results of this study indicate the proposed method is an efficient tool to evaluate the public health risk associated with crayfish consumption.
马杜霉素（MAD）被广泛引入水生环境，导致鱼产品受到污染。令人担忧的是，食用受MAD污染的小龙虾可能会引发哈夫病症状。在本研究中，为监测这种潜在污染并了解MAD在小龙虾可食用组织中的残留和消除特性，开发、验证并应用了一种灵敏高效的超高效液相色谱 - 串联质谱法。用乙腈提取并经固相萃取柱净化后，采用正离子模式的多反应监测质谱法测定MAD的残留量。检测限和定量限分别为6 μg·kg和20 μg·kg。加标回收率在74.2%至110.4%之间，相对标准偏差为1.2%至10.1%。此外，MAD分别在3天和5天后从小龙虾的腹肌和肝胰腺组织中完全消除。MAD在肌肉中的最大残留限量（MRLs）分别为200 μg·kg，在肝胰腺中为600 μg·kg，其在两种可食用组织中的休药期为25.8 °C·d。总体而言，本研究结果表明所提出的方法是评估与食用小龙虾相关的公共卫生风险的有效工具。
