Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University 28108(Unesp), São José dos Campos, São Paulo, Brazil.
Universidade Anhembi Morumbi, São José dos Campos, São Paulo, Brazil.
Hum Exp Toxicol. 2021 Aug;40(8):1374-1382. doi: 10.1177/0960327121991904. Epub 2021 Feb 5.
Tobacco use may initiate the process of oral carcinogenesis with clinically undetectable changes. Smoking cessation may prevent its progression. The objective of this study was to evaluate the association between DNA ploidy and micronucleus (MN) frequency in chronic smokers. Three groups were evaluated: Smoker Group, Former Smoker Group and Control Group. Exfoliative cytology was performed on the lateral border of the tongue and mouth floor. MN and DNA ploidy analyses were performed, as well as the correlation between the variables. The data showed a difference between the groups for the total MN (p = 0.0227), and the Smoker group had the highest mean (4.22 ± 4.12). The three groups did not differ statistically from each other on ploidy evaluation (p-value > 0.05). There was also an association between aneuploidy and increased MN frequency in the Former Smoker group (p = 0.0036). In conclusion, these results point out that there is a relationship between the frequency of MN and aneuploidy in former smokers. Moreover, smoking cessation, even for a short period of time, may promote the decrease of MN frequency caused by tobacco use.
吸烟可能会引发口腔癌的发生，而这种变化在临床上是无法检测到的。戒烟可能会阻止其进展。本研究的目的是评估 DNA 倍体与慢性吸烟者微核（MN）频率之间的关系。评估了三组人群：吸烟者组、曾经吸烟者组和对照组。在舌侧缘和口腔底部进行脱落细胞学检查。进行了 MN 和 DNA 倍体分析，并对变量之间的相关性进行了分析。数据显示，总 MN 在组间存在差异（p=0.0227），吸烟者组的平均值最高（4.22±4.12）。三组在倍体评估上无统计学差异（p 值>0.05）。在曾经吸烟者组中，非整倍体与 MN 频率增加之间也存在关联（p=0.0036）。总之，这些结果表明，MN 频率与曾经吸烟者的非整倍体之间存在关系。此外，即使是短时间的戒烟也可能会降低吸烟引起的 MN 频率的增加。
