Klug C, Baltes W, Krönert W, Weber R
Institut für Lebensmittelchemie der Technischen Universität Berlin.
Z Lebensm Unters Forsch. 1988 Feb;186(2):108-13. doi: 10.1007/BF01042702.
A suitable method has been developed for the routine analysis of the ergot alkaloids ergometrine, ergometrinine, ergosine, ergosinine, ergotamine, ergotaminine, ergocornine, ergocorninine, alpha-ergocryptine, alpha-ergocryptinine, beta-ergocryptine, beta-ergocryptinine, ergocristine and ergocristinine in cereal products. The method consists of food extraction, cleaning of the crude extract by a modified form of the Extrelut method, and identification and quantitative determination of the alkaloids by high pressure liquid chromatography (HPLC). The results are confirmed by thin layer chromatography (TLC) and gas-chromatography/mass spectrometry (GC/MS). Market investigations have shown contaminations in ecological as well as in conventional products, with rye products mainly being contaminated. Within the EEC, a maximum value of 0.05% ergot respectively a total alkaloid content of 1 mg/kg in cereals used for food production is prescribed. This value was not exceeded in any of the investigated samples.
已开发出一种适用于常规分析谷物产品中麦角生物碱麦角新碱、麦角异新碱、麦角胺、麦角异胺、麦角克碱、麦角异克碱、α-麦角隐亭、α-麦角隐亭宁、β-麦角隐亭、β-麦角隐亭宁、麦角晶碱和麦角晶宁的方法。该方法包括食品提取、采用改良的埃特拉路特法对粗提取物进行净化，以及通过高压液相色谱法（HPLC）对生物碱进行鉴定和定量测定。结果通过薄层色谱法（TLC）和气-相色谱/质谱联用仪（GC/MS）进行确认。市场调查显示，生态产品和传统产品中均存在污染，其中黑麦产品受污染情况最为严重。在欧盟内部，规定用于食品生产的谷物中麦角的最大值为0.05%，或总生物碱含量为1毫克/千克。在所调查的任何样品中均未超过该值。
