Food and Nutrition Department, School of Food Engineering, University of Campinas - UNICAMP, R Monteiro Lobato, Campinas, Brazil.
Institute of Health and Biotechnology, Federal University of Amazonas - UFAM, Estrada Coari-Mamiá, 305, Coari, AM, 69460-000, Brazil.
J Sci Food Agric. 2021 Sep;101(12):4907-4915. doi: 10.1002/jsfa.11134. Epub 2021 Feb 22.
Pot-pollen is a fermented product stored by stingless bees in cerumen pots and traditionally used as food or medicine by natives in tropical regions. Knowledge of pot-pollen composition from the Amazon region is important to strengthen the breeding of native bees and consequently contribute to sustainable development in this region. The aim of the present study was to determine the chemical composition of Melipona seminigra pot-pollen from Amazonas, Brazil.
We report the identification of 21 phenolic compounds using ultra-performance liquid chromatography- electrospray ionization-quadrupole time-of-flight-mass spectrometry. Overall, the predominant constituents of pot-pollen were protein (333.8-470.7 g kg ) and dietary fibers (247.6-353.3 g kg ). The predominant fatty acids were polyunsaturated (44.95-59.57% of total fatty acid content) and the samples contained all essential amino acids. Total carotenoid content ranged from 3.2 to 48.0 μg g , total flavonoid content (as catechin equivalents) from 1.9 to 4.5 mg g , total reducing capacity (as gallic acid equivalents) from 7.0 to 15.0 mg g , ferric-reducing antioxidant power from 2.8 to 8.9 μmol g and oxygen radical absorbance capacity (as Trolox equivalents) from 224.9 to 1117.0 μmol g .
This study reports for the first time a comprehensive chemical characterization of M. seminigra pot-pollen. The samples presented antioxidant activity, high values of nutrients and bioactive compounds such as polyphenols, carotenoids, insoluble dietary fibers, polyunsaturated fatty acids, and essential amino acids, comparable to other health foods. © 2021 Society of Chemical Industry.
蜂花粉是无刺蜜蜂在蜡腺中储存的发酵产物，传统上被热带地区的原住民用作食物或药物。了解亚马逊地区的蜂花粉成分对于加强本土蜜蜂的养殖具有重要意义，从而有助于该地区的可持续发展。本研究的目的是确定巴西亚马逊地区 Melipona seminigra 蜂花粉的化学成分。
我们使用超高效液相色谱-电喷雾电离-四极杆飞行时间质谱法鉴定了 21 种酚类化合物。总体而言，蜂花粉的主要成分是蛋白质（333.8-470.7 g kg）和膳食纤维（247.6-353.3 g kg）。主要脂肪酸为多不饱和脂肪酸（占总脂肪酸含量的 44.95-59.57%），且样品中含有所有必需氨基酸。类胡萝卜素总含量范围为 3.2-48.0 μg g，总黄酮含量（以儿茶素当量计）为 1.9-4.5 mg g，总还原能力（以没食子酸当量计）为 7.0-15.0 mg g，铁还原抗氧化能力为 2.8-8.9 μmol g，氧自由基吸收能力（以 Trolox 当量计）为 224.9-1117.0 μmol g。
本研究首次全面描述了 M. seminigra 蜂花粉的化学成分。这些样品具有抗氧化活性，且含有高价值的营养成分和生物活性化合物，如多酚、类胡萝卜素、不溶性膳食纤维、多不饱和脂肪酸和必需氨基酸，与其他健康食品相当。© 2021 英国化学学会。
