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委内瑞拉梅里达蜂花粉四种颜色组分的抗氧化活性

Antioxidant activity of four color fractions of bee pollen from Mérida, Venezuela.

作者信息

Pérez-Pérez Elizabeth M, Vit Patricia, Rivas Efraín, Sciortino Rosa, Sosa Angel, Tejada Daniel, Rodríguez-Malaver Antonio J

机构信息

Departamento de Bioanálisis Clinico, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida 5101, Venezuela.

出版信息

Arch Latinoam Nutr. 2012 Dec;62(4):375-80.

PMID:24020258
Abstract

Bee pollen has been reported to show antioxidant and radical scavenging activities; contributing to anti-inflammatory and gastroprotective properties. Venezuelan honeybee pollen has been little studied, but is consumed because its properties are known from other countries reports. On the basis of these reports, water, ethanol and methanol soluble fractions were prepared from dried bee-pollen commercially available and produced by La Montaña farm (Mérida, Venezuela). These fractions were evaluated for their functional properties, specifically, polyphenol content and total antioxidant activity. Pollen samples were separated by color in four fractions: yellow, brown, orange and ochre. Polyphenol content ranged between 396.7 to 1286.7 gallic acid equivalents GAE/100 g pollen; it was highest in pollen homogenates obtained with ethanol, followed by those obtained with methanol and water. The antioxidant activity ranged from 0.50 to 1.84 micromoles Trolox equivalents TEAC/100 g for water and ethanol homogenates respectively. The results presented in this work suggest that the ethanol extract of bee pollen show a potent antioxidant activity, comparable to human plasma, probably due to total polyphenol content of bee pollen. This is important because the bee pollen would be beneficial not only as a dietary supplement but also as a functional food.

摘要

据报道,蜂花粉具有抗氧化和自由基清除活性,有助于抗炎和胃保护特性。委内瑞拉蜜蜂花粉的研究较少,但因其在其他国家报道中所具有的特性而被食用。基于这些报道,从委内瑞拉梅里达市拉蒙塔纳农场生产的市售干蜂花粉中制备了水、乙醇和甲醇可溶级分。对这些级分的功能特性进行了评估,特别是多酚含量和总抗氧化活性。花粉样品按颜色分为四个级分:黄色、棕色、橙色和赭色。多酚含量在396.7至1286.7没食子酸当量GAE/100 g花粉之间;用乙醇获得的花粉匀浆中含量最高,其次是用甲醇和水获得的花粉匀浆。水和乙醇匀浆的抗氧化活性分别为0.50至1.84微摩尔Trolox当量TEAC/100 g。这项工作的结果表明,蜂花粉的乙醇提取物具有强大的抗氧化活性,与人体血浆相当,这可能归因于蜂花粉中的总多酚含量。这很重要,因为蜂花粉不仅作为膳食补充剂有益,而且作为功能性食品也有益。

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