Department of Joint Orthopaedics, Weifang Traditional Chinese Hospital, Weifang, Shandong, China.
Department of Joint Surgery, Weifang People's Hospital, Weifang, Shandong, China.
J Biol Regul Homeost Agents. 2021 Jan-Feb;35(1):151-160. doi: 10.23812/20-695-A.
Osteosarcoma (OS) is the most frequent primary malignancy in bone, and commonly occurs in children and adolescents. The aim of this study was to assess the aberrant expression of miR-1274a in OS patients, and to evaluate the role of miR-1274a as a prognostic biomarker and tumor suppressor in OS progression. miR-1274a expression was estimated using quantitative real-time polymerase chain reaction. The Kaplan-Meier method and Cox regression analysis were used to evaluate the prognostic value of miR-1274a in OS. Gain- and loss-of-function in vitro experiments were used to explore the functional role of miR-1274a in OS progression. A target gene of miR-1274a was analyzed using a dual-luciferase reporter assay. miR-1274a expression was decreased in OS tissues and associated with distant metastasis and clinical stages in OS patients. Low miR-1274a could predict poor overall survival and disease-free survival in OS. The overexpression of miR-1274a could inhibit OS cell proliferation, migration and invasion. Additionally, ADAM9 was demonstrated to serve as a direct target of miR-1274a in OS cells. In conclusion, reduced miR-1274a predicts poor prognosis and serves as a potential tumor suppressor in OS. ADAM9 is a target of miR-1274a, which may mediate the functional role of miR-1274a in OS progression.
骨肉瘤（OS）是最常见的原发性骨恶性肿瘤，常见于儿童和青少年。本研究旨在评估 miR-1274a 在 OS 患者中的异常表达，并评估 miR-1274a 作为 OS 进展的预后生物标志物和肿瘤抑制因子的作用。采用实时定量聚合酶链反应（PCR）估计 miR-1274a 的表达。采用 Kaplan-Meier 法和 Cox 回归分析评估 miR-1274a 在 OS 中的预后价值。采用体外 gain-和 loss-of-function 实验探索 miR-1274a 在 OS 进展中的功能作用。采用双荧光素酶报告基因检测分析 miR-1274a 的靶基因。miR-1274a 在 OS 组织中表达降低，与 OS 患者的远处转移和临床分期相关。低 miR-1274a 可预测 OS 患者总生存期和无病生存期不良。miR-1274a 的过表达可抑制 OS 细胞的增殖、迁移和侵袭。此外，ADAM9 被证明是 OS 细胞中 miR-1274a 的直接靶基因。总之，miR-1274a 的下调预示着预后不良，可作为 OS 中的潜在肿瘤抑制因子。ADAM9 是 miR-1274a 的靶基因，可能介导 miR-1274a 在 OS 进展中的功能作用。
