Department of Comprehensive Orthopedics, Linyi People's Hospital, Linyi, 276000, Shandong, China.
Prison Hospital, Linyi People's Hospital, Linyi, 276000, Shandong, China.
J Orthop Surg Res. 2020 Oct 2;15(1):454. doi: 10.1186/s13018-020-01967-1.
Osteosarcoma (OS) is one of the most primary malignant bone tumors, mainly attracting children and young adults. The microRNAs are mentioned to play vital roles in many cancers, including OS. The purpose of this study was to explore the expression and function of miR-455-3p in OS and predict the potential effects in clinical diagnosis and prognosis.
We conducted quantitative real-time PCR to assess the expression of miR-455-3p in OS tissues and cell lines. The Cell Counting Kit-8 assay, Transwell assay, and flow cytometry were performed to assess the ability of miR-455-3p on cell proliferation, migration, invasion, and apoptosis. Kaplan-Meier curve and Cox regression analysis were used to demonstrate the survival outcome.
This study revealed that the expression of miR-455-3p was decreased in OS tissues and cell lines. The dysregulation of miR-455-3p was in association with tumor size, distant metastasis, and clinical stage. Patients with high miR-455-3p expression had a satisfying survival rate. Multivariate Cox analysis indicated that miR-455-3p was a promising prognostic indicator. Expression of miR-455-3p could inhibit the proliferation, migration, and invasion, and facilitate apoptosis of OS cells in vitro.
These results indicated the miR-455-3p was a potential clinical therapeutic target and prognostic biomarker by suppressing the proliferation, migration, and invasion, as well as enhancing cell apoptosis.
骨肉瘤（OS）是最常见的原发性恶性骨肿瘤之一，主要发生在儿童和青少年。有研究表明微小 RNA（miRNA）在许多癌症中发挥着重要作用，包括骨肉瘤。本研究旨在探讨 miR-455-3p 在骨肉瘤中的表达和功能，并预测其在临床诊断和预后中的潜在作用。
我们采用实时定量 PCR 检测 miR-455-3p 在骨肉瘤组织和细胞系中的表达。通过细胞计数试剂盒-8 检测、Transwell 检测和流式细胞术评估 miR-455-3p 对细胞增殖、迁移、侵袭和凋亡的影响。Kaplan-Meier 曲线和 Cox 回归分析用于评估生存结果。
本研究表明 miR-455-3p 在骨肉瘤组织和细胞系中表达下调。miR-455-3p 的失调与肿瘤大小、远处转移和临床分期有关。miR-455-3p 高表达的患者具有较好的生存率。多因素 Cox 分析表明 miR-455-3p 是一种有前途的预后指标。miR-455-3p 的表达可以抑制骨肉瘤细胞的增殖、迁移和侵袭，并促进细胞凋亡。
这些结果表明 miR-455-3p 通过抑制增殖、迁移和侵袭，促进细胞凋亡，可能成为一种有潜力的临床治疗靶点和预后生物标志物。
