Centre of Obstetrics and Gynecology, Faculty of Medicine, Institute of Clinical Medicine, Vilnius University, Vilnius, Lithuania.
Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Faculty of Medicine, Institute of Biomedical Sciences, Vilnius University, Vilnius, Lithuania.
Arch Gynecol Obstet. 2021 Jul;304(1):157-162. doi: 10.1007/s00404-021-05976-1. Epub 2021 Feb 5.
To evaluate the agreement of wet smear microscopy with Gram stain microscopy and to assess whether it is possible to predict Mycoplasmas/Ureaplasmas when analysing vaginal secretion with Gram stain and wet smear microscopy.
Women with complaints of the abnormal vaginal discharge were invited to participate. A sample of vaginal secretion was taken for wet smear microscopy and for Gram staining analysis. A sample from the endocervical canal was taken for DNA detection of seven infections: Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis. The percentage agreement between wet smear and Gram stain was determined and the Cohen's Kappa values were calculated.
Of 158 consecutive women included, one (or a few) of the infections were detected in 54% of them and the most frequent infection was Ureaplasma parvum (79% of all the cases with infections). The percentage agreement between vaginal wet smear and Gram stain was 73% (Cohen's Kappa value 0.63). A statistically significant association between the DNA detected Mycoplasmas/Ureaplasmas and bacterial vaginosis was found (positive amine test p = 0.046, wet smear p = 0.005 and Gram stain p = 0.03).
There was a statistically significant association between bacterial vaginosis and the DNA detected Mycoplasmas/Ureaplasmas. The agreement of vaginal wet smear with Gram stain was good.
评估湿涂片显微镜检查与革兰氏染色显微镜检查的一致性，并评估在分析阴道分泌物时进行革兰氏染色和湿涂片显微镜检查是否可以预测支原体/脲原体。
邀请有异常阴道分泌物症状的女性参加。采集阴道分泌物进行湿涂片显微镜检查和革兰氏染色分析。从宫颈内口采集样本，用于七种感染的 DNA 检测：人型支原体、生殖支原体、解脲脲原体、解脲脲原体、沙眼衣原体、淋病奈瑟菌和阴道毛滴虫。确定湿涂片和革兰氏染色之间的百分比一致性，并计算 Cohen's Kappa 值。
在 158 名连续女性中，54%的女性检测到一种（或几种）感染，最常见的感染是解脲脲原体（所有感染病例的 79%）。阴道湿涂片和革兰氏染色之间的百分比一致性为 73%（Cohen's Kappa 值为 0.63）。在 DNA 检测到的支原体/脲原体和细菌性阴道病之间发现了统计学上显著的关联（阳性胺试验 p=0.046，湿涂片 p=0.005，革兰氏染色 p=0.03）。
细菌性阴道病与 DNA 检测到的支原体/脲原体之间存在统计学显著关联。阴道湿涂片与革兰氏染色的一致性良好。
