School of Pharmacy, Health Science Center, Xi'an Jiaotong University, No. 76, Yanta West Road, Xi'an, 710061, China.
Department of Pharmacy, Shenzhen Baoan Authentic TCM Therapy Hospital, Shenzhen, 518101, China.
Top Curr Chem (Cham). 2021 Feb 5;379(2):10. doi: 10.1007/s41061-020-00323-5.
Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.
荧光成像是生物医学领域的一种重要方法。荧光成像是一种非破坏性的方法，具有高效率和高灵敏度、高分辨率以及能够实时动态监测活细胞的优点。然而，它也存在一些缺点，例如背景信号高和选择性低。生物正交反应具有非破坏性和有效性的优点，用于追踪和分析体内的生物相互作用。本综述重点介绍了近年来对荧光探针作用机制的理解的进展。
