Department of Cardiovascular Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China.
Biosensors (Basel). 2024 Jul 8;14(7):333. doi: 10.3390/bios14070333.
Bioanalysis based on optical imaging has gained significant progress in the last few decades. Luminescence probes are capable of detecting, monitoring, and tracing particular biomolecules in complex biological systems to figure out the roles of these molecules in organisms. Considering the rapid development of luminescence probes for bio-applications and their promising future, we have attempted to explore the working principles and recent advances in bio-applications of luminescence probes, in the hope of helping readers gain a detailed understanding of luminescence probes developed in recent years. In this review, we first focus on the current widely used luminescence probes, including fluorescence probes, bioluminescence probes, chemiluminescence probes, afterglow probes, photoacoustic probes, and Cerenkov luminescence probes. The working principles for each type of luminescence probe are concisely described and the bio-application of the luminescence probes is summarized by category, including metal ions detection, secretion detection, imaging, and therapy.
基于光学成像的生物分析在过去几十年中取得了重大进展。荧光探针能够在复杂的生物体系中检测、监测和追踪特定的生物分子，以了解这些分子在生物体中的作用。鉴于生物应用中荧光探针的快速发展及其广阔的前景，我们尝试探讨了荧光探针的工作原理及其在生物应用中的最新进展，希望帮助读者详细了解近年来开发的荧光探针。在这篇综述中，我们首先集中讨论了目前广泛使用的荧光探针，包括荧光探针、生物发光探针、化学发光探针、余晖探针、光声探针和切伦科夫辐射发光探针。简要描述了每种类型荧光探针的工作原理，并按类别总结了荧光探针的生物应用，包括金属离子检测、分泌检测、成像和治疗。
