School of Biomedical Engineering, International University,Quarter 6, Linh Trung, Thu Duc District, Ho Chi Minh City, Viet Nam.
and Vietnam National University, Ho Chi Minh City, Viet Nam.
Biol Chem. 2020 Oct 19;402(2):155-165. doi: 10.1515/hsz-2019-0402. Print 2021 Jan 27.
Fibrillar fibronectin (FFN), an active form of fibronectin (FN), plays important roles in various cellular processes. Our goal is to investigate effect of FFN morphology on cellular behaviors. Plasma FN at two concentrations was cross-linked into FFN by dialysis against 2 M urea followed by morphological analysis under Scanning Electron Microscopy. To evaluate effect of FFN morphology, fibroblasts were cultured on FN or different FFNs. Fibroblast behaviors including adhesion, spreading, and migration were evaluated. Our data showed that FN fibrillogenesis was dependent on FN concentration. At high concentrations (0.75 mg/mL), large FFN approximately 2.167 + 0.875 µm in diameter were formed with attached nodular structures and rough surface. In contrast, smooth surface FFN fibrils with diameter of 1.886 + 0.412 µm were formed from FN at 0.25 mg/mL. Cellular assays revealed morphological dependent biological effects of different FFNs. Fibroblast separately adhered to native FN and remained spherical while on FFN, cells attached with higher quantity and showed spreading morphology. A synergistic ligand interaction of integrin α5β1 and αvβ3 was observed in cell adhering on FFN. Cell migration results showed that large FFN decreased migration rate while small FFN did not. Taken together, our data draws new attention towards controlling biological function of FN by its fibrillar structure.
纤维连接蛋白（FN）的纤维状形式（FFN）是 FN 的一种活性形式，在各种细胞过程中发挥着重要作用。我们的目标是研究 FFN 形态对细胞行为的影响。通过用 2M 尿素透析将两种浓度的血浆 FN 交联成 FFN，然后在扫描电子显微镜下进行形态分析。为了评估 FFN 形态的影响，将成纤维细胞培养在 FN 或不同的 FFN 上。评估了成纤维细胞的行为，包括黏附、铺展和迁移。我们的数据表明 FN 纤维形成依赖于 FN 的浓度。在高浓度（0.75mg/ml）下，形成了直径约为 2.167+0.875µm 的大 FFN，带有附着的结节结构和粗糙表面。相比之下，在 0.25mg/ml 的 FN 下形成了直径为 1.886+0.412µm 的光滑表面 FFN 纤维。细胞测定显示，不同 FFN 的形态依赖性生物学效应。成纤维细胞分别黏附在天然 FN 上并保持球形，而在 FFN 上，细胞黏附的数量更多，并呈现出铺展的形态。在细胞黏附在 FFN 上时，观察到整合素 α5β1 和 αvβ3 的协同配体相互作用。细胞迁移结果表明，大 FFN 降低了迁移率，而小 FFN 则没有。总之，我们的数据引起了人们对通过 FN 的纤维状结构来控制 FN 生物学功能的新关注。
