Kazemi Modje, Jafarzadeh Abdollah, Nemati Maryam, Taghipour Fereshteh, Oladpour Omolbanin, Rezayati Mohammad Taghi, Khorramdelazad Hossain, Hassan Zuhair Mohammad
Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Islamic Republic of Iran.
Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Islamic Republic of Iran.
J Complement Integr Med. 2021 Feb 1;18(2):303-310. doi: 10.1515/jcim-2019-0135.
The potent anti-tumorigenic effects were attributed to ginger and there are some reports regarding the anti-cancer and immunomodulatory properties ginger-derived components. This study aimed to investigate the effects of zingerone on some immune-related parameters in an animal model of breast cancer.
The breast cancer was established in female BALB/c mice using a carcinogenic 4T1 cell line. At day 10 after cancer induction, tumor-bearing mice were divided into five groups and treated intraperitoneal (daily from days 11-30) with saline or zingerone (at doses 10, 20, 50 and 100 mg/kg/day). The mice were sacrificed on day 31 and the number of splenic Th1- and Treg cells, the expression of IFN-γ and TGF-β in the blood mononuclear cells, the antibody production against sheep red blood cell (SRBC) were determined using flow cytometry, real time-PCR and a standard hemagglutination assay, respectively.
Zingerone at doses 50 and 100 mg/kg enhanced the number of splenic Th1 cells (p<0.03 and 0.007, respectively); at doses 10, 20, 50 and 100 mg/kg reduced the number of splenic Treg cells (p<0.02, 0.01, and 0.01, respectively), at doses 50 and 100 mg/kg enhanced the expression of IFN-γ (p<0.03), at doses 50 and 100 mg/kg reduced the expression of TGF-β, at doses 50 mg/kg reduced the titer of anti-SRBC antibody (p<0.05).
Zingerone improve the T cell-mediated and antibody responses in a mouse model of breast cancer. The immunotherapeutic potentials of zingerone in cancers need more considerations.
姜具有强大的抗肿瘤作用，且有一些关于姜衍生成分的抗癌和免疫调节特性的报道。本研究旨在探讨姜辣素对乳腺癌动物模型中一些免疫相关参数的影响。
使用致癌性4T1细胞系在雌性BALB/c小鼠中建立乳腺癌模型。在诱导癌症后第10天，将荷瘤小鼠分为五组，腹腔注射（从第11天至30天每日一次）生理盐水或姜辣素（剂量分别为10、20、50和100mg/kg/天）。在第31天处死小鼠，分别使用流式细胞术、实时PCR和标准血凝试验测定脾Th1细胞和调节性T细胞（Treg）数量、血液单核细胞中IFN-γ和TGF-β的表达、针对绵羊红细胞（SRBC）的抗体产生情况。
50和100mg/kg剂量的姜辣素可增加脾Th1细胞数量（分别为p<0.03和0.007）；10、20、50和100mg/kg剂量可减少脾Treg细胞数量（分别为p<0.02、0.01和0.01），50和100mg/kg剂量可增加IFN-γ的表达（p<0.03），50和100mg/kg剂量可降低TGF-β的表达，50mg/kg剂量可降低抗SRBC抗体效价（p<0.05）。
姜辣素可改善乳腺癌小鼠模型中T细胞介导的反应和抗体反应。姜辣素在癌症中的免疫治疗潜力需要更多的研究。
