Department of Microbiology, China Medical University, Taichung, Taiwan.
Food Chem. 2013 Jan 1;136(1):170-7. doi: 10.1016/j.foodchem.2012.07.124. Epub 2012 Aug 10.
Ginger is a commonly used spice with anti-inflammatory potential. Colitis is the common pathological lesion of inflammatory bowel diseases. In this study, we investigated the therapeutic effects of ginger and its component zingerone in mice with 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Ginger and zingerone ameliorated TNBS-induced colonic injury in a dose-dependent manner. Pathway analysis of ginger- and zingerone-regulated gene expression profiles showed that ginger and zingerone significantly regulated cytokine-related pathways. Network analysis showed that nuclear factor-κB (NF-κB) and interleukin-1β (IL-1β) were key molecules involved in the expression of ginger- and zingerone-affected genes. Ex vivo imaging and immunohistochemical staining further verified that ginger and zingerone suppressed TNBS-induced NF-κB activation and IL-1β protein level in the colon. In conclusion, ginger improved TNBS-induced colitis via modulation of NF-κB activity and IL-1β signalling pathway. Moreover, zingerone might be the active component of ginger responsible for the amelioration of colitis induced by TNBS.
生姜是一种常用的香料，具有抗炎潜力。结肠炎是炎症性肠病的常见病理病变。在这项研究中，我们研究了生姜及其成分姜酮在 2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎小鼠中的治疗作用。生姜和姜酮以剂量依赖的方式改善了 TNBS 诱导的结肠损伤。生姜和姜酮调节的基因表达谱的途径分析表明，生姜和姜酮显著调节细胞因子相关途径。网络分析表明，核因子-κB（NF-κB）和白细胞介素-1β（IL-1β）是涉及生姜和姜酮影响基因表达的关键分子。离体成像和免疫组织化学染色进一步证实，生姜和姜酮抑制了 TNBS 诱导的 NF-κB 激活和结肠中 IL-1β 蛋白水平。总之，生姜通过调节 NF-κB 活性和 IL-1β 信号通路改善了 TNBS 诱导的结肠炎。此外，姜酮可能是生姜中负责改善 TNBS 诱导的结肠炎的活性成分。
