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含生姜提取物的聚乙二醇化纳米脂质体对荷结直肠癌小鼠的抗肿瘤作用。

Anti-tumor effects of PEGylated-nanoliposomes containing ginger extract in colorectal cancer-bearing mice.

作者信息

Yavari Maryam, Jaafari Mahmoud Reza, Mirzavi Farshad, Mosayebi Ghasem, Ghazavi Ali, Ganji Ali

机构信息

Department of Immunology & Microbiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Iran J Basic Med Sci. 2022 Jul;25(7):890-896. doi: 10.22038/IJBMS.2022.63870.14075.

DOI:10.22038/IJBMS.2022.63870.14075
PMID:36033959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9392564/
Abstract

OBJECTIVES

This study aimed to develop a nanoliposomal formulation containing ginger ethanolic extract with a higher therapeutic effect for cancer treatment.

MATERIALS AND METHODS

The present study aimed to prepare PEGylated nanoliposomal ginger through the thin film hydration method plus extrusion. Physicochemical characteristics were evaluated, and the toxicity of the prepared liposomes was assessed using the MTT assay. In addition, tumor size was monitored in colorectal cancer-bearing mice. Also, the anticancer effects of liposomal ginger were evaluated by gene expression assay of Bax and Bcl-2 and cytokines including TNF-α, TGF-β, and IFN-γ by Real-time PCR. Also, cytotoxic T lymphocytes (CTLs) and regulatory T lymphocytes (Treg cells) were counted in spleen and tumor tissue by flow cytometry assay.

RESULTS

The nanoliposomes' particle size and polydispersity index (PDI) were 94.95 nm and 0.246 nm, respectively. High encapsulation capacity (80 %) confirmed the technique's efficiency, and the release rate of the extract was 85% at pH 6.5. In addition, this study showed that liposomal ginger at 100 mg/kg/day enhanced the expression of Bax (0.05) and IFN-γ (0.01) compared with ginger extract in the mouse model. Also, the number of tumor-infiltrating lymphocytes (TILs) and CTLs cell count in tumor tissue showed a significant increase in the LipGin group compared with the Gin group (0.05).

CONCLUSION

Results indicated that the liposomal ginger enhanced the antitumor activity; therefore, the prepared liposomal ginger can be used in future clinical trials.

摘要

目的

本研究旨在开发一种含有生姜乙醇提取物的纳米脂质体制剂，用于癌症治疗，具有更高的治疗效果。

材料与方法

本研究旨在通过薄膜水化法加挤压法制备聚乙二醇化纳米脂质体生姜。评估其理化特性，并使用MTT法评估所制备脂质体的毒性。此外，监测荷结直肠癌小鼠的肿瘤大小。同时，通过实时PCR对Bax和Bcl-2以及包括TNF-α、TGF-β和IFN-γ在内的细胞因子进行基因表达分析，评估脂质体生姜的抗癌效果。此外，通过流式细胞术分析对脾脏和肿瘤组织中的细胞毒性T淋巴细胞（CTL）和调节性T淋巴细胞（Treg细胞）进行计数。

结果

纳米脂质体的粒径和多分散指数（PDI）分别为94.95nm和0.246nm。高包封率（80%）证实了该技术的有效性，提取物在pH 6.5时的释放率为85%。此外，本研究表明，在小鼠模型中，与生姜提取物相比，100mg/kg/天的脂质体生姜可增强Bax（P<0.05）和IFN-γ（P<0.01）的表达。此外，与生姜组相比，脂质体生姜组肿瘤组织中肿瘤浸润淋巴细胞（TIL）数量和CTL细胞计数显著增加（P<0.05）。

结论

结果表明脂质体生姜增强了抗肿瘤活性；因此，所制备的脂质体生姜可用于未来的临床试验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/7f6d5991104d/IJBMS-25-890-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/d65cd2c2294e/IJBMS-25-890-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/b9f66dcac0b2/IJBMS-25-890-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/afb8de1599a5/IJBMS-25-890-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/5281cc19f71b/IJBMS-25-890-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/5ef0c7a1a584/IJBMS-25-890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/1e7d6514e12f/IJBMS-25-890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/0de4039a5570/IJBMS-25-890-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/de403214d5b1/IJBMS-25-890-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/7f6d5991104d/IJBMS-25-890-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/d65cd2c2294e/IJBMS-25-890-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/b9f66dcac0b2/IJBMS-25-890-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/afb8de1599a5/IJBMS-25-890-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/5281cc19f71b/IJBMS-25-890-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/5ef0c7a1a584/IJBMS-25-890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/1e7d6514e12f/IJBMS-25-890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/0de4039a5570/IJBMS-25-890-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/de403214d5b1/IJBMS-25-890-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6f6/9392564/7f6d5991104d/IJBMS-25-890-g009.jpg

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