Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Sao Paulo, 14040-901, Brazil.
National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), UNESP, Institute of Chemistry, Araraquara, Sao Paulo, 14800-900, Brazil.
Electrophoresis. 2021 Sep;42(17-18):1726-1743. doi: 10.1002/elps.202000334. Epub 2021 Feb 15.
Enantioresolution processes are vital tools for investigating the enantioselectivities of chiral compounds. An analyst resolves to optimize enantioresolution conditions once they are determined. Generally, optimization is conducted by a one-factor-at-a-time (OFAT) approach. Although this approach may determine an adequate condition for the method, it does not often allow the estimation of the real optimum condition. Experimental designs are conducive for the optimization of enantioresolution methods via capillary electromigration techniques (CETs). They can efficiently extract information from the behavior of a method and enable the estimation of the real optimum condition. Furthermore, the application of the analytical quality by design (AQbD) approach to the development of CET-based enantioselective methods is a trend. This article (i) offers an overview of the application of experimental designs to the development of enantioselective methods from 2015 to mid-2020, (ii) reveals the experimental designs that are presently employed in CET-based enantioresolutions, and (iii) offers a critical point of view on how the different experimental designs can aid the optimization of enantioresolution processes by considering the method parameters.
对映体拆分过程是研究手性化合物对映选择性的重要工具。一旦确定了对映体拆分条件，分析人员就会进行优化。通常，通过单因素逐一（OFAT）方法进行优化。尽管这种方法可以为方法确定一个足够的条件，但它通常无法估计真实的最优条件。实验设计有利于通过毛细管电泳技术（CET）优化对映体拆分方法。它们可以有效地从方法的行为中提取信息，并能够估计真实的最优条件。此外，将分析质量源于设计（AQbD）方法应用于基于 CET 的手性选择性方法的开发是一种趋势。本文（i）概述了 2015 年至 2020 年年中实验设计在开发对映选择性方法中的应用，（ii）揭示了目前在基于 CET 的对映体拆分中使用的实验设计，以及（iii）考虑到方法参数，从如何通过不同的实验设计帮助优化对映体拆分过程的角度提出了一个关键观点。
