用于手性分离优化的实验设计方法：综述

Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview.

作者信息

Klein-Júnior Luiz Carlos, Mangelings Debby, Vander Heyden Yvan

机构信息

Pharmaceutical Chemistry Research Group, Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.

Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium.

出版信息

Methods Mol Biol. 2019;1985:453-478. doi: 10.1007/978-1-4939-9438-0_27.

DOI:10.1007/978-1-4939-9438-0_27

PMID:31069752

Abstract

In this chapter, the application of design of experiments (DoE) for chiral separation optimization using supercritical fluid chromatography (SFC), liquid chromatography (LC), capillary electrophoresis (CE), and capillary electrochromatography (CEC) methods is reviewed. Both screening and optimization steps are covered, including a discussion of each aspect, such as factor-, level-, and response selection. Different designs are also presented, highlighting their applications.

摘要

在本章中，综述了实验设计（DoE）在使用超临界流体色谱（SFC）、液相色谱（LC）、毛细管电泳（CE）和毛细管电色谱（CEC）方法进行手性分离优化中的应用。涵盖了筛选和优化步骤，包括对各个方面的讨论，如因子、水平和响应的选择。还介绍了不同的设计，并突出了它们的应用。