沙雷氏游动放线菌重组复苏促进因子蛋白，大口黑鲈（Micropterus salmoides）有前景的疫苗候选物。
Recombinant resuscitation-promoting factor protein of Nocardia seriolae, a promissing vaccine candidate for largemouth bass (Micropterus salmoides).
机构信息
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung, 91201, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung, 91201, Taiwan; Southern Taiwan Fish Disease Centre, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung, 91201, Taiwan; International Degree Program of Ornamental Fish Science and Technology, International College, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung, 91201, Taiwan.
出版信息
Fish Shellfish Immunol. 2021 Apr;111:127-139. doi: 10.1016/j.fsi.2021.01.015. Epub 2021 Feb 2.
Nocardia seriolae is an important pathogenic bacterium that causes nocardiosis in various fish species and leads to economic losses in the fish industry. To develop an effective subunit vaccine against nocardial infection, the truncated resuscitation-promoting factor (tRPF) of N. seriolae was selected and recombinantly produced using the Escherichia coli expression system. Western blotting results indicated that the recombinant protein could be strongly recognised by largemouth bass anti-N. seriolae antibodies. The protective efficacy of tRPF recombinant protein was assessed in combination with the commercial adjuvant Montanide™ ISA 763 A VG. The results showed that emulsified tRPF + ISA significantly induced high serum antibody response and serum lysozyme activity in the vaccinated fish. Quantitative reverse transcription polymerase chain reaction analysis indicated that tRPF + ISA could notably enhance the expression of immune-related genes in both the head kidney and spleen of the vaccinated fish. Finally, vaccinated largemouth bass displayed higher immuno-protection with a relative percent survival of 69.23% compared to the control groups. Taken together, the combination of tRPF + ISA is an ideal vaccine candidate against N. seriolae infection.
沙雷氏诺卡氏菌是一种重要的致病性细菌，可引起各种鱼类的诺卡氏病，给鱼类养殖业造成经济损失。为了开发针对诺卡氏菌感染的有效亚单位疫苗，选择了沙雷氏诺卡氏菌的截断复苏促进因子（tRPF），并使用大肠杆菌表达系统进行了重组生产。Western blot 结果表明，重组蛋白可被大口黑鲈抗沙雷氏诺卡氏菌抗体强烈识别。将 tRPF 重组蛋白与商业佐剂 Montanide™ ISA 763 A VG 联合评估其保护效力。结果表明，乳化的 tRPF+ISA 可显著诱导接种鱼类产生高血清抗体反应和血清溶菌酶活性。定量逆转录聚合酶链反应分析表明，tRPF+ISA 可显著增强接种鱼头肾和脾脏中免疫相关基因的表达。最后，与对照组相比，接种大口黑鲈的相对存活率为 69.23%，表现出更高的免疫保护力。综上所述，tRPF+ISA 的组合是一种针对沙雷氏诺卡氏菌感染的理想疫苗候选物。
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