Center for Endemic Disease Control, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Harbin, 150081, China.
Center for Endemic Disease Control, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Harbin, 150081, China.
Environ Toxicol Pharmacol. 2021 May;84:103604. doi: 10.1016/j.etap.2021.103604. Epub 2021 Feb 2.
People who drink water contaminated with arsenic for a long time develop neuritis, cerebellar symptoms, and deficits in memory and intellectual function. Arsenic induces oxidative stress and promotes apoptosis through multiple signalling pathways in nerve cells. Neuroglobin (Ngb), as a key mediator, is considered to be protective against oxidative stress. In this study, we aimed to study the effects of Ngb knockdown in arsenite-treated rat neurons on levels of apoptosis markers and reactive oxygen species and serum Ngb levels of subjects from arsenic-endemic regions in China. We discovered that arsenic-induced apoptosis and reactive oxygen species production were enhanced in Ngb-knocked-down rat neurons. Silencing of Ngb aggravated the arsenic-induced decrease in the rate of Bcl-2/Bax and the levels of Bcl-2 protein following arsenite treatment. The results also showed that serum Ngb levels were independently negatively correlated with arsenic concentration in drinking water. Furthermore, the serum Ngb levels of four groups (245 individuals) according to different degree exposure to arsenic were 815.18 ± 89.52, 1247.97 ± 117.18, 774.79 ± 91.55, and 482.72 ± 49.30 pg/mL, respectively. Taken together, it can be deduced that Ngb has protective effects against arsenic-induced apoptosis by eliminating reactive oxygen species.
长期饮用砷污染水的人会患上神经炎、小脑症状以及记忆力和智力功能障碍。砷通过多种信号通路在神经细胞中诱导氧化应激并促进细胞凋亡。神经球蛋白（Ngb）作为关键介质，被认为对氧化应激具有保护作用。在这项研究中，我们旨在研究 Ngb 敲低对亚砷酸盐处理的大鼠神经元中细胞凋亡标志物和活性氧水平以及中国砷疫区人群血清 Ngb 水平的影响。我们发现，砷诱导的细胞凋亡和活性氧生成在 Ngb 敲低的大鼠神经元中增强。沉默 Ngb 加重了亚砷酸盐处理后 Bcl-2/Bax 比率和 Bcl-2 蛋白水平下降。结果还表明，血清 Ngb 水平与饮用水中砷浓度呈独立负相关。此外，根据不同程度暴露于砷的四组（245 人）的血清 Ngb 水平分别为 815.18±89.52pg/ml、1247.97±117.18pg/ml、774.79±91.55pg/ml 和 482.72±49.30pg/ml。综上所述，可以推断 Ngb 通过消除活性氧对砷诱导的细胞凋亡具有保护作用。
