Department of Chemistry & Biochemistry, University of California, Santa Barbara, CA 93106, United States; Department of Physics, University of California, Santa Barbara, CA 93106, United States.
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
Curr Opin Struct Biol. 2021 Apr;67:219-225. doi: 10.1016/j.sbi.2020.12.012. Epub 2021 Feb 2.
Intrinsically disordered proteins (IDPs) are an important class of proteins that do not fold to a well-defined three-dimensional shape but rather adopt an ensemble of inter-converting conformations. This feature makes their experimental characterization challenging and invites a theoretical and computational approach to complement experimental studies. In this review, we highlight the recent progress in developing new computational and theoretical approaches to study the structure and dynamics of monomeric and order higher assemblies of IDPs, with a particular emphasis on their phase separation into protein-rich condensates.
无规卷曲蛋白质（IDPs）是一类重要的蛋白质，它们不会折叠成明确的三维形状，而是采用一系列相互转化的构象。这一特性使得它们的实验特征变得具有挑战性，并需要一种理论和计算方法来补充实验研究。在这篇综述中，我们强调了最近在开发新的计算和理论方法来研究 IDPs 的单体和更高阶组装的结构和动力学方面的进展，特别强调了它们向富含蛋白质的凝聚相的相分离。
