Nantong University Xinglin College, Nantong, China.
Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovatioin Center of Neuroregeneration, Nantong University, Nantong, China. Email:
Ann Palliat Med. 2021 Jan;10(1):625-632. doi: 10.21037/apm-20-2580.
Salvia miltiorrhiza is a traditional Chinese medicine with remarkable antioxidant, antibacterial, and anticoagulant properties. In the present study, we investigated the effects of Salvia miltiorrhiza injection in protecting Schwann cells (SCs) from hydrogen peroxide (H2O2)-induced cell apoptosis.
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence staining were used to detect the establishment of the SC apoptosis model induced by H2O2. The effect of Salvia miltiorrhiza injection on injured cell morphology was observed, and the effect on cell apoptosis was determined by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Western blotting analysis was used to detect the effect of Salvia miltiorrhiza injection on apoptosis-related protein expression.
The results of the MTT assay showed that cell activity significantly decreased after treatment with 1 mM H2O2, but different concentrations of Salvia miltiorrhiza injection could improve cell activity at different degrees. The number of cells increased significantly after treatment with Salvia miltiorrhiza injection. Annexin V-FITC/PI double staining and TUNEL results revealed that Salvia miltiorrhiza injection could significantly reduce apoptosis induced by H2O2. Western blotting analysis showed that the expression of Bcl-2 was significantly upregulated, while the expression level of Bax was significantly downregulated.
Salvia miltiorrhiza injection can protect SCs from H2O2-induced cell apoptosis, and has potential therapeutic effects in neurological disease.
丹参是一种具有显著抗氧化、抗菌和抗凝特性的中药。本研究旨在探讨丹参注射液对过氧化氢（H2O2）诱导施万细胞（SCs）凋亡的保护作用。
采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和免疫荧光染色法建立 H2O2 诱导的SCs 凋亡模型。观察丹参注射液对损伤细胞形态的影响，采用 Annexin V-荧光素异硫氰酸酯（FITC）凋亡检测试剂盒和末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记（TUNEL）法检测细胞凋亡情况。Western blot 分析检测丹参注射液对凋亡相关蛋白表达的影响。
MTT 检测结果显示，1mM H2O2 处理后细胞活性明显降低，但不同浓度的丹参注射液可在不同程度上提高细胞活性。丹参注射液处理后细胞数量明显增加。Annexin V-FITC/PI 双染和 TUNEL 结果显示，丹参注射液可显著减少 H2O2 诱导的细胞凋亡。Western blot 分析显示，Bcl-2 表达明显上调，而 Bax 表达水平明显下调。
丹参注射液可保护SCs 免受 H2O2 诱导的细胞凋亡，在神经疾病中具有潜在的治疗作用。
