CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111784. doi: 10.1016/j.msec.2020.111784. Epub 2020 Dec 10.
Ohmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca. The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced changes in the functional properties of both gel forming solutions and final scaffolds (contact angle, swelling, porosity, compressive modulus and degradation rate). The scaffolds' biological performance was evaluated regarding their ability to support the adhesion and proliferation of human fibroblast cells. The production process resulted in a non-cytotoxic material and the changes imposed by the presence of the EF during the scaffolds' production improved cellular proliferation and metabolic activity. Protein functionalization assisted by OH presents a promising new alternative for the production of improved and tuneable protein-based scaffolds for tissue engineering.
欧姆加热（OH）被认为是一种新兴的加工技术，由于其能够诱导和控制蛋白质功能，最近受到越来越多的关注。在这项研究中，OH 首次用于组织工程支架的生产。BSA/酪蛋白溶液通过 OH 进行处理，促进蛋白质变性和聚集，然后通过添加 Ca 进行冷凝胶化。通过 OH 处理时的温度和处理时间，形成稳定的支架。电场（EF）的变化引起凝胶形成溶液和最终支架的功能特性的变化（接触角、溶胀、孔隙率、压缩模量和降解率）。支架的生物性能根据其支持人成纤维细胞黏附和增殖的能力进行评估。该生产工艺产生了一种非细胞毒性材料，并且 EF 在支架生产过程中存在所引起的变化提高了细胞的增殖和代谢活性。OH 辅助的蛋白质功能化提供了一种有前途的新方法，可用于生产用于组织工程的改良和可调谐的蛋白质支架。
