Marbio, Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT-The Arctic University of Norway, N-9037 Tromsø, Norway.
Microalgae and Microbiomes, Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT-The Arctic University of Norway, N-9037 Tromsø, Norway.
Mar Drugs. 2021 Feb 3;19(2):87. doi: 10.3390/md19020087.
"One strain many compounds" (OSMAC) based approaches have been widely used in the search for bioactive compounds. Introducing stress factors like nutrient limitation, UV-light or cocultivation with competing organisms has successfully been used in prokaryote cultivation. It is known that diatom physiology is affected by changed cultivation conditions such as temperature, nutrient concentration and light conditions. Cocultivation, though, is less explored. Hence, we wanted to investigate whether grazing pressure can affect the metabolome of the marine diatom , and if the stress reaction could be detected as changes in bioactivity. cultures were mass cultivated in large volume bioreactor (6000 L), first as a monoculture and then as a coculture with live zooplankton. Extracts of the diatom biomass were screened in a selection of bioactivity assays: inhibition of biofilm formation, antibacterial and cell viability assay on human cells. Bioactivity was found in all bioassays performed. The viability assay towards normal lung fibroblasts revealed that had higher bioactivity when cocultivated with zooplankton than in monoculture. Cocultivation with diatoms had no noticeable effect on the activity against biofilm formation or bacterial growth. The metabolic profiles were analyzed showing the differences in diatom metabolomes between the two culture conditions. The experiment demonstrates that grazing stress affects the biochemistry of and thus represents a potential tool in the OSMAC toolkit.
“一种菌株，多种化合物”（OSMAC）方法已广泛应用于寻找生物活性化合物。在原核生物培养中，引入营养限制、紫外线或与竞争生物共培养等应激因素已被成功应用。已知硅藻的生理学受培养条件的变化影响，如温度、营养浓度和光照条件。然而，共培养的研究较少。因此，我们想研究摄食压力是否会影响海洋硅藻的代谢组，如果应激反应可以作为生物活性的变化来检测。我们在大型生物反应器（6000 L）中大规模培养，首先是单一培养，然后是与活体浮游动物的共培养。硅藻生物量的提取物在一系列生物活性测定中进行筛选：生物膜形成抑制、对人类细胞的抗菌和细胞活力测定。在所有进行的生物测定中都发现了生物活性。对正常肺成纤维细胞的活力测定表明，与浮游动物共培养时， 的生物活性高于单一培养。与硅藻共培养对生物膜形成或细菌生长的活性没有明显影响。分析代谢谱显示两种培养条件下硅藻代谢组的差异。该实验表明，摄食压力会影响的生物化学特性，因此它代表了 OSMAC 工具包中的一个潜在工具。
