Nilusha Rathmalgodage Thejani, Wei Yuansong
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environment Technology Section, Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 07 00700, Sri Lanka.
Membranes (Basel). 2021 Feb 3;11(2):108. doi: 10.3390/membranes11020108.
Cake layer formation is an inevitable challenge in membrane bioreactor (MBR) operation. The investigations on the cake layer microbial community are essential to control biofouling. This work studied the bacterial and archaeal communities in the cake layer, the anaerobic sludge, and the membrane cleaning solutions of anaerobic membrane bioreactor (AnMBR) with yttria-based ceramic tubular membrane by polymerase chain reaction (PCR) amplification of 16S rRNA genes. The cake layer resistance was 69% of the total membrane resistance. Proteins and soluble microbial by-products (SMPs) were the dominant foulants in the cake layer. The pioneering archaeal and bacteria in the cake layer were mostly similar to those in the anaerobic bulk sludge. The dominant biofouling bacteria were , , , and and the dominant archaeal were and at family level. This finding may help to develop antifouling membranes for AnMBR treating domestic wastewater.
在膜生物反应器（MBR）运行过程中，滤饼层的形成是一个不可避免的挑战。对滤饼层微生物群落的研究对于控制生物污染至关重要。本研究通过对16S rRNA基因进行聚合酶链反应（PCR）扩增，研究了基于氧化钇的陶瓷管式膜厌氧膜生物反应器（AnMBR）的滤饼层、厌氧污泥和膜清洗液中的细菌和古菌群落。滤饼层阻力占总膜阻力的69%。蛋白质和可溶性微生物副产物（SMPs）是滤饼层中的主要污染物。滤饼层中最初出现的古菌和细菌大多与厌氧混合污泥中的相似。在科水平上，主要的生物污染细菌为 、 、 和 ，主要的古菌为 和 。这一发现可能有助于开发用于AnMBR处理生活污水的抗污染膜。
