Tayab Mohammed Abu, Chowdhury Kazi Ashfak Ahmed, Jabed Md, Mohammed Tareq Syed, Kamal A T M Mostafa, Islam Mohammad Nazmul, Uddin A M Kafil, Hossain Mohammad Adil, Emran Talha Bin, Simal-Gandara Jesus
Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh.
Plants (Basel). 2021 Feb 3;10(2):287. doi: 10.3390/plants10020287.
Dhaiphul () is a frequently demanded plant in South-East Asian regions for its diverse medicinal values. This study was proposed to examine antioxidant, antidiabetic, and antidepressant potentials of methanol extract of leaves (MEWF) and its derived n-hexane (NHFMEWF) and ethyl acetate (EAFMEWF) fractions through in vitro, in vivo, and computational models. Among test samples, MEWF and EAFMEWF contained the highest phenolic content and showed maximal antioxidant activity in DPPH radical scavenging and ferric reducing power assays. In comparison, NHFMEWF possessed maximum flavonoid content and a significantly potent α-amylase inhibitory profile comparable with positive control acarbose. In animal models of depression (forced swimming and tail suspension test), EAFMEWF and NHFMEWF demonstrated a dose-dependent antidepressant-like effect; explicitly, the depressive-like behaviors significantly declined in EAFMEWF-treated dosing groups in contrast to the control group. In the computational analysis, previously isolated flavonoid compounds from Dhaiphul leaves manifested potent binding affinity against several key therapeutic target proteins of diabetes and depressive disorders including α-amylase, serotonin transporter, dopamine transporter, and neuronal nitric oxide synthase with varying pharmacokinetics and toxicity profiles. This research's outcomes may provide potential dietary supplements for mitigating hyperglycemia, cellular toxicity, and depressive disorder.
Dhaiphul（）是东南亚地区因具有多种药用价值而经常被需求的植物。本研究旨在通过体外、体内和计算模型研究其叶甲醇提取物（MEWF）及其衍生的正己烷（NHFMEWF）和乙酸乙酯（EAFMEWF）馏分的抗氧化、抗糖尿病和抗抑郁潜力。在测试样品中，MEWF和EAFMEWF的酚类含量最高，在DPPH自由基清除和铁还原能力测定中表现出最大的抗氧化活性。相比之下，NHFMEWF的黄酮类含量最高，其α-淀粉酶抑制作用明显强于阳性对照阿卡波糖。在抑郁症动物模型（强迫游泳和悬尾试验）中，EAFMEWF和NHFMEWF表现出剂量依赖性的抗抑郁样作用；具体而言，与对照组相比，EAFMEWF处理的给药组中抑郁样行为显著减少。在计算分析中，先前从Dhaiphul叶中分离出的黄酮类化合物对糖尿病和抑郁症的几种关键治疗靶点蛋白表现出强大的结合亲和力，包括α-淀粉酶、5-羟色胺转运体、多巴胺转运体和神经元型一氧化氮合酶，具有不同的药代动力学和毒性特征。本研究结果可能为缓解高血糖、细胞毒性和抑郁症提供潜在的膳食补充剂。
