Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Department of Woman, Child and General and Special Surgery, University of Campania "Luigi Vanvitelli", 80138 Napoli, Italy.
Cells. 2021 Feb 3;10(2):310. doi: 10.3390/cells10020310.
The aquatic environment is involved in the pollutants spreading mechanisms, including nanomaterials and heavy metals. The aims of this study were to assess the in vivo genotoxicity of Cd (1 mg/L) and to investigate the genomic effects generated by its co-exposure with TiO-NPs (10 µg/L). The study was performed using zebrafish as a model for 5, 7, 14, 21, and 28 days of exposure. The genotoxic potential was assessed by three experimental approaches: DNA integrity, degree of apoptosis, and molecular alterations at the genomic level by genomic template stability (% GTS) calculation. Results showed an increased in DNA damage after Cd exposure with a decrease in % GTS. The co-exposure (TiO-NPs + Cd) induced a no statistically significant loss of DNA integrity, a reduction of the apoptotic cell percentage and the recovery of genome stability for prolonged exposure days. Characterization and analytical determinations data showed Cd adsorption to TiO-NPs, which reduced free TiO-NPs levels. The results of our study suggest that TiO-NPs could be used for the development of controlled heavy metal bioremediation systems.
水生环境参与污染物的传播机制，包括纳米材料和重金属。本研究的目的是评估 Cd（1mg/L）的体内遗传毒性，并研究其与 TiO-NPs（10μg/L）共同暴露产生的基因组效应。该研究使用斑马鱼作为模型，进行了 5、7、14、21 和 28 天的暴露实验。通过三种实验方法评估遗传毒性潜力：DNA 完整性、细胞凋亡程度和基因组水平的分子变化，通过基因组模板稳定性（%GTS）计算。结果表明，Cd 暴露后 DNA 损伤增加，%GTS 降低。共同暴露（TiO-NPs+Cd）在延长的暴露天数内并未导致 DNA 完整性明显丧失、凋亡细胞比例降低和基因组稳定性恢复。表征和分析测定数据表明，Cd 吸附到 TiO-NPs 上，降低了游离 TiO-NPs 的水平。我们的研究结果表明，TiO-NPs 可用于开发控制重金属生物修复系统。
