Rodríguez-Villarreal Angeles Ivón, Carmona-Flores Manuel, Colomer-Farrarons Jordi
Department of Electronics and Biomedical Engineering, Faculty of Physics, University of Barcelona, 08028 Barcelona, Spain.
Membranes (Basel). 2021 Feb 3;11(2):109. doi: 10.3390/membranes11020109.
Blood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental results of red blood cell (RBC) flow, suspended in a microfluidic device, while temperature and flow rate incrementally modify RBC response within the microflow. Studies of human red blood cell flow, at a concentration of 20%, suspended in its autologous plasma and phosphate-buffered saline (PBS) buffer, were carried out at a wide flow rate, varying between 10 and 230 μL/min and a temperature range of 23 °C to 50 °C. The plotted measures show an increment in a CFA near the channel wall due to cell flow inertia after a constricted channel, which becomes more significant as temperature and flow rate increase. The temperature increment widened the CFA up to three times. In comparison, flow rate increment increased the CFA up to 20 times in PBS and 11 times in plasma.
在微器件中对血细胞进行操控是一项有趣的任务，可通过颗粒的大小、密度来分离颗粒，或者将它们从悬浮的缓冲液中去除，以便进行进一步分析等等。本研究基于悬浮在微流控装置中的红细胞（RBC）流动的实验结果，突出了无细胞区域（CFA）的扩大，同时温度和流速会逐渐改变微流中红细胞的反应。对浓度为20%、悬浮于自身血浆和磷酸盐缓冲盐水（PBS）缓冲液中的人类红细胞流动进行了研究，流速范围为10至230μL/分钟，温度范围为23°C至50°C。绘制的测量结果显示，在通道变窄后，由于细胞流动惯性，通道壁附近的CFA会增加，并且随着温度和流速的升高，这种增加会变得更加显著。温度升高使CFA扩大了三倍。相比之下，流速增加使PBS中的CFA扩大了20倍，血浆中的CFA扩大了11倍。
