Calejo Joana, Pinho Diana, Galindo-Rosales Francisco J, Lima Rui, Campo-Deaño Laura
ESTiG, Polytechnic Institute of Bragança, C. Sta Apolónia, 5301-857 Bragança, Portugal.
CEFT, Faculty of Engineering of the University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Micromachines (Basel). 2015 Dec 30;7(1):4. doi: 10.3390/mi7010004.
The interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies related to the use of particulate solutions in which the particles mimic the behaviour of the red blood cells (RBCs) or erythrocytes. One of the most relevant effects related with the behaviour of the erythrocytes is a cell free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen in microcirculatory environments. Recent studies have shown that the CFL enhancement is possible with an insertion of contraction and expansion region in a straight microchannel. These effects are useful for cell manipulation or sorting in lab-on-chip studies. In this experimental study we present particulate Newtonian and non-Newtonian solutions which resulted in a rheological blood analogue able to form a CFL, downstream of a microfluidic hyperbolic contraction, in a similar way of the one formed by healthy RBCs.
由于实验性血液动力学研究数量的增加以及由于伦理、经济或危险问题在处理真实血液时所面临的困难，近年来对血液替代品开发的兴趣一直在增加。尽管在文献中可以找到单相牛顿和非牛顿血液替代品，但很少有研究涉及使用颗粒溶液，其中颗粒模拟红细胞（RBC）或红血球的行为。与红细胞行为相关的最相关效应之一是无细胞层（CFL）的形成，这包括红细胞向血管中心迁移，在血管壁附近形成细胞耗尽的血浆区域，这在微循环环境中是已知会发生的。最近的研究表明，通过在直的微通道中插入收缩和扩张区域，可以增强CFL。这些效应对于芯片实验室研究中的细胞操作或分选很有用。在本实验研究中，我们展示了颗粒牛顿和非牛顿溶液，它们在微流体双曲线收缩下游形成了一种能够形成CFL的流变血液替代品，其方式与健康红细胞形成的类似。
