伊朗美沙酮反应者与无反应者成瘾者中CYP2B6基因rs3745274和rs3211371多态性的研究
Investigating the CYP2B6 rs3745274 and rs3211371 polymorphisms in Methadone-Responder and Non-Responder Addicts in Iran.
作者信息
Sadat Aghabozorg Afjeh Sara, Boshehri Behzad, Hamednia Safar, Amini Asmaolhosna, Mashayekhi Parisa, Omrani Mir Davood
机构信息
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Forensic Medicine and Toxicology, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
Iran Biomed J. 2021 Feb 6;25(3):220-5. doi: 10.29252/ibj.25.3.220.
BACKGROUND
Methadone therapy is a major protocol in opioid addiction cases in many health care systems. Population-based studies have shown that in addicted people, the genetic profile affects their response to methadone therapy. Therefore, this study designed to examine the frequency of two SNPs of the CYP2B6 gene (rs3745274 and rs3211371) in addicted cases in two methadone-responders and methadone non-responders groups.
METHODS
A total of 199 opioid-addicted individuals and 117 unaffected control subjects were genotyped for rs3745274 and rs3211371 polymorphisms of the CYP2B6 gene using the tetra-primer amplification refractory mutation system-polymerase chain reaction.
RESULTS
Results of this study revealed the significant association of rs3745274 GG (p < 0.001; OR = 0.027; 95% CI = 0.14-0.49) and GT (p < 0.001; OR = 4.04; 95% CI = 2.26-7.21) genotypes with the risk of addiction in methadone-responders. Also, a significant association between rs3745274 GG (p < 0.001; OR = 0.28; 95% CI = 0.15-0.51) and GT (p < 0.001; OR = 5.1; 95% CI = 2.8-5.28) genotypes and addiction relapse was found in methadone non-responders.
CONCLUSION
Based on our findings, we can conclude that rs3745274 variant of CYP2B6 gene could serve as a potential biomarker, to evaluate the prognosis of addicted people fate under treatment with methadone.
背景
在许多医疗保健系统中，美沙酮疗法是治疗阿片类药物成瘾病例的主要方案。基于人群的研究表明，成瘾者的基因特征会影响他们对美沙酮疗法的反应。因此，本研究旨在检测细胞色素P450 2B6（CYP2B6）基因的两个单核苷酸多态性（SNP，rs3745274和rs3211371）在两组对美沙酮有反应者和无反应者成瘾病例中的频率。
方法
使用四引物扩增阻滞突变系统-聚合酶链反应对总共199名阿片类药物成瘾个体和117名未受影响的对照受试者进行CYP2B6基因rs3745274和rs3211371多态性基因分型。
结果
本研究结果显示，rs3745274基因的GG（p<0.001；比值比[OR]=0.027；95%置信区间[CI]=0.14 - 0.49）和GT（p<0.001；OR = 4.04；95%CI = 2.26 - 7.21）基因型与美沙酮反应者的成瘾风险显著相关。此外，在美沙酮无反应者中发现rs3745274基因的GG（p<0.001；OR = 0.28；95%CI = 0.15 - 0.51）和GT（p<0.001；OR = 5.1；95%CI = 2.8 - 5.28）基因型与成瘾复发之间存在显著关联。
结论
基于我们的研究结果，可以得出结论，CYP2B6基因的rs3745274变异体可作为一种潜在的生物标志物，用于评估接受美沙酮治疗的成瘾者的预后。
Zotero 插件