Moran-Bertot Ivis, Rodríguez-Cabrera Lianet, Borras-Hidalgo Orlando, Huang Siliang, Kan Yunchao, Wright Denis J, Ayra-Pardo Camilo
Plant Division, Centre for Genetic Engineering and Biotechnology (CIGB), 10600, Havana, Cuba.
Shandong Provincial Key Laboratory of Microbial Engineering, School of Biotechnology, Qi Lu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
BMC Plant Biol. 2021 Feb 5;21(1):78. doi: 10.1186/s12870-021-02854-5.
The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae.
The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls.
We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.
先前有报道称小菜蛾的PxSDF2L1基因在高基础转录率下可增强昆虫对病原体的抗性。PxSDF2L1与基质细胞衍生因子2（SDF2）相似，SDF2是一种内质网应激诱导的伴侣蛋白，在动植物中高度保守。SDF2的确切生物学功能尚不清楚，但其表达是植物先天免疫所必需的。在此，我们研究在本氏烟草中持续表达PxSDF2L1是否同样能赋予对植物病原体的抗性，特别是对烟草黑胫病菌烟草疫霉变种的抗性。
用携带PxSDF2L1基因的基于PVX的二元载体转化的农杆菌接种本氏烟草植株；以携带绿色荧光蛋白（GFP）基因的PVX载体进行类似的农杆菌接种实验作为对照。在盆栽试验中，与对照相比，组成型表达PxSDF2L1的农杆菌感染的本氏烟草植株接种烟草疫霉后茎部病害症状显著减轻。
我们证实了PxSDF2L1在抗烟草黑胫病中的作用，其在商业烟草品种中对这种卵菌工程化主动抗性方面具有潜在应用价值，并建议在其他作物家族和植物病原体中对其进行评估。
