Key Laboratory of Tobacco Biotechnological Breeding, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, China.
State Key Laboratory for Conservation and Utilization of Bio-Resource in Yunnan, Yunnan University, Kunming, 650021, China.
Sci Rep. 2017 Mar 24;7(1):401. doi: 10.1038/s41598-017-00481-5.
Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and environmental conditions. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare transcriptional changes in the stems of tobacco upon inoculation with P. nicotianae strain race 0. We used two tobacco varieties: RBST (named from resistance to black shank and tobacco mosaic virus), which was resistant to the P. nicotianae strain race 0, and Honghuadajinyuan (HD), which was susceptible to P. nicotianae race 0. Samples were collected 12 and 72-hour post inoculation (hpi). Analysis of differentially expressed genes (DEGs) and significantly enriched GO terms indicated that several basic defense mechanisms were suppressed in both varieties, which included response to wounding (GO: 0009611), and defense response to fungus (GO: 0050832). We also found some genes that may especially be related to mechanisms of resistance in RBST, such as the one encoding a chitinase. These results will provide a valuable resource for understanding the interactions between P. nicotianae and tobacco plants.
黑胫病是由烟草疫霉（Phytophthora nicotianae）引起的，是一种严重的世界性栽培烟草病害。烟草与烟草疫霉之间的相互作用较为复杂，相互作用的结果取决于烟草基因型、烟草疫霉菌株和环境条件。在本研究中，我们使用 RNA 测序（RNA-Seq）技术研究并比较了接种烟草疫霉菌株 0 型后烟草茎部的转录变化。我们使用了两个烟草品种：RBST（因对黑胫病和烟草花叶病毒的抗性而得名），对烟草疫霉菌株 0 型具有抗性，以及红花大金源（HD），对 P. nicotianae 菌株 0 型敏感。样品分别在接种后 12 小时和 72 小时采集。差异表达基因（DEGs）和显著富集 GO 术语的分析表明，两种品种中的几种基本防御机制均受到抑制，包括对创伤的反应（GO：0009611）和对真菌的防御反应（GO：0050832）。我们还发现了一些可能与 RBST 中抗性机制特别相关的基因，如编码几丁质酶的基因。这些结果将为理解烟草疫霉与烟草植物之间的相互作用提供有价值的资源。
