Faculty of Biology, Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Faculty of Biology, Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
FEMS Microbiol Ecol. 2021 Feb 5;97(2). doi: 10.1093/femsec/fiaa259.
This study analyzed the occurrence of lanthanide-dependent (XoxF type) methanol dehydrogenases in the bacterial community dominated by Proteobacteria inhabiting shale rock. In total, 22 sequence matches of XoxF were identified in the metaproteome of the community. This enzyme was produced by bacteria represented by orders such as Rhizobiales, Rhodobacterales, Rhodospiralles, Burkholderiales and Nitrosomonadales. Among the identified XoxF proteins, seven belonged to XoxF1 clade and 15 to XoxF5 clade. This study is the first to show the occurrence of XoxF proteins in the metaproteome of environmental lithobiontic bacterial community colonizing an underground rock rich in light lanthanides. The presented results broaden our understanding of the ecology of XoxF producing bacteria as well as the distribution and diversity of these enzymes in the natural environment.
本研究分析了在以栖居于页岩岩石的变形菌门为主导的细菌群落中，镧依赖性（XoxF 型）甲醇脱氢酶的发生情况。在群落的宏蛋白质组中总共鉴定出 22 个 XoxF 的序列匹配。这种酶由根瘤菌目、红杆菌目、红螺旋菌目、伯克霍尔德菌目和硝化单胞菌目等细菌产生。在鉴定出的 XoxF 蛋白中，有 7 种属于 XoxF1 分支，15 种属于 XoxF5 分支。本研究首次表明 XoxF 蛋白存在于富轻镧地下岩石中环境生石生物细菌群落的宏蛋白质组中。所呈现的结果拓宽了我们对产生 XoxF 的细菌的生态学以及这些酶在自然环境中的分布和多样性的理解。
