miR-3178 作为胃癌的预后指标和肿瘤抑制因子。

miR-3178 as a prognostic indicator and tumor suppressor of gastric cancer.

机构信息

Department of General Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361001, Fujian, China.

Department of Laboratory, Weifang People's Hospital, Weifang, 261000, Shandong, China.

出版信息

Ir J Med Sci. 2022 Feb;191(1):139-145. doi: 10.1007/s11845-021-02527-0. Epub 2021 Feb 5.

DOI:10.1007/s11845-021-02527-0

PMID:33547612

Abstract

BACKGROUND

Gastric cancer is a common malignant tumor with an increasing death rate. MicroRNA can serve as a promising biomarker for the progression and prognosis of various cancers.

AIMS

The clinical significance and biological function of miR-3178 in gastric cancer was assessed in this study.

METHODS

A total of 117 paired tissues were collected from gastric cancer patients. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-3178 in gastric cancer tissues and cells. The association between miR-3178 expression and the clinicopathological features of patients were analyzed by χ test. Kaplan-Meier analysis and Cox regression were employed to investigate the prognostic value of miR-3178. Finally, the effect of miR-3178 on the cellular process of gastric cancer was investigated by CCK-8 and transwell assay.

RESULTS

miR-3178 was downregulated in gastric cancer tissues and cells, which showed a significant association with the TNM stage and lymph node metastasis of patients and a poor prognosis. MiR-3178 and TNM stage were considered as two independent prognostic factors for gastric cancer. Furthermore, the downregulation of miR-3178 promoted cell proliferation, migration, and invasion of gastric cancer by regulating Notch1.

CONCLUSION

miR-3178 may be involved in the progression of gastric cancer, which provides new insights into the treatment of gastric cancer.

摘要

背景

胃癌是一种常见的恶性肿瘤，死亡率不断上升。微小 RNA 可以作为各种癌症进展和预后的有前途的生物标志物。

目的

本研究评估了 miR-3178 在胃癌中的临床意义和生物学功能。

方法

收集了 117 对胃癌患者的组织。采用实时定量聚合酶链反应检测胃癌组织和细胞中 miR-3178 的表达。通过卡方检验分析 miR-3178 表达与患者临床病理特征的关系。采用 Kaplan-Meier 分析和 Cox 回归分析探讨 miR-3178 的预后价值。最后，通过 CCK-8 和 Transwell 检测研究 miR-3178 对胃癌细胞过程的影响。