Department of General Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361001, Fujian, China.
Department of Laboratory, Weifang People's Hospital, Weifang, 261000, Shandong, China.
Ir J Med Sci. 2022 Feb;191(1):139-145. doi: 10.1007/s11845-021-02527-0. Epub 2021 Feb 5.
Gastric cancer is a common malignant tumor with an increasing death rate. MicroRNA can serve as a promising biomarker for the progression and prognosis of various cancers.
The clinical significance and biological function of miR-3178 in gastric cancer was assessed in this study.
A total of 117 paired tissues were collected from gastric cancer patients. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-3178 in gastric cancer tissues and cells. The association between miR-3178 expression and the clinicopathological features of patients were analyzed by χ test. Kaplan-Meier analysis and Cox regression were employed to investigate the prognostic value of miR-3178. Finally, the effect of miR-3178 on the cellular process of gastric cancer was investigated by CCK-8 and transwell assay.
miR-3178 was downregulated in gastric cancer tissues and cells, which showed a significant association with the TNM stage and lymph node metastasis of patients and a poor prognosis. MiR-3178 and TNM stage were considered as two independent prognostic factors for gastric cancer. Furthermore, the downregulation of miR-3178 promoted cell proliferation, migration, and invasion of gastric cancer by regulating Notch1.
miR-3178 may be involved in the progression of gastric cancer, which provides new insights into the treatment of gastric cancer.
胃癌是一种常见的恶性肿瘤，死亡率不断上升。微小 RNA 可以作为各种癌症进展和预后的有前途的生物标志物。
本研究评估了 miR-3178 在胃癌中的临床意义和生物学功能。
收集了 117 对胃癌患者的组织。采用实时定量聚合酶链反应检测胃癌组织和细胞中 miR-3178 的表达。通过卡方检验分析 miR-3178 表达与患者临床病理特征的关系。采用 Kaplan-Meier 分析和 Cox 回归分析探讨 miR-3178 的预后价值。最后，通过 CCK-8 和 Transwell 检测研究 miR-3178 对胃癌细胞过程的影响。
miR-3178 在胃癌组织和细胞中下调，与患者的 TNM 分期和淋巴结转移显著相关，且预后不良。miR-3178 和 TNM 分期被认为是胃癌的两个独立预后因素。此外，miR-3178 的下调通过调节 Notch1 促进了胃癌细胞的增殖、迁移和侵袭。
miR-3178 可能参与了胃癌的进展，为胃癌的治疗提供了新的思路。
