Liu Fengli, Cui Jiyan, Xue Dongyun
Department of Gastroenterology, Shandong Provincial Third Hospital, Shandong University.
Tohoku J Exp Med. 2023 Sep 12;261(1):13-23. doi: 10.1620/tjem.2023.J009. Epub 2023 Feb 16.
Gastric cancer is a common malignant tumor with a relatively poor prognosis after surgery. Non-coding RNA may serve as biomarkers for the progression and prognosis of various cancers. The clinical significance and biological function of lncRNA TRHDE-AS1 and miR-1275 in gastric cancer were assessed in this study. 119 paired tissues were selected with adequate clinical information. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression level of lncRNA TRHDE-AS1 and miR-1275 in gastric cancer tissues and cells. The association between lncRNA TRHDE-AS1 or miR-1275 expression and the clinicopathological features of patients was analyzed by the Pearson Chi-square test. Kaplan-Meier analysis and Multi-variate Cox proportional regression analysis were utilized to evaluate the prognostic value of lncRNA TRHDE-AS1 and miR-1275. Finally, the effect of TRHDE-AS1 binding to miR-1275 on the gastric cancer cellular process was investigated by CCK-8 and Transwell assay. LncRNA TRHDE-AS1 was found to be downregulated in gastric cancer tissues and cells, but miR-1275 upregulated, which both showed significant associations with clinical pathology of gastric cancer patients (including TNM stage and lymph node metastasis) and a poor prognosis. LncRNA TRHDE-AS1 and miR-1275 can be considered two independent prognostic factors for gastric cancer. Furthermore, the upregulation of lncRNA TRHDE-AS1 inhibited cell proliferation, migration, and invasion of gastric cancer partly by miR-1275. LncRNA TRHDE-AS1/miR-1275 axis may be involved in the progression of gastric cancer and can be promising prognostic factors, which may provide new insights into the treatment of gastric cancer.
胃癌是一种常见的恶性肿瘤，术后预后相对较差。非编码RNA可作为各种癌症进展和预后的生物标志物。本研究评估了长链非编码RNA TRHDE-AS1和miR-1275在胃癌中的临床意义和生物学功能。选取了119对具有充分临床信息的组织。采用定量实时聚合酶链反应（qRT-PCR）检测长链非编码RNA TRHDE-AS1和miR-1275在胃癌组织和细胞中的表达水平。通过Pearson卡方检验分析长链非编码RNA TRHDE-AS1或miR-1275表达与患者临床病理特征之间的关联。采用Kaplan-Meier分析和多变量Cox比例回归分析评估长链非编码RNA TRHDE-AS1和miR-1275的预后价值。最后，通过CCK-8和Transwell实验研究TRHDE-AS1与miR-1275结合对胃癌细胞进程的影响。发现长链非编码RNA TRHDE-AS1在胃癌组织和细胞中表达下调，而miR-1275表达上调，二者均与胃癌患者的临床病理（包括TNM分期和淋巴结转移）及不良预后显著相关。长链非编码RNA TRHDE-AS1和miR-1275可被视为胃癌的两个独立预后因素。此外，长链非编码RNA TRHDE-AS1的上调部分通过miR-1275抑制胃癌细胞的增殖、迁移和侵袭。长链非编码RNA TRHDE-AS1/miR-1275轴可能参与胃癌的进展，有望成为预后因素，这可能为胃癌的治疗提供新的见解。
