Cornford E M
Southwest-Regional VA Epilepsy Center, Veterans Administration Wadsworth Medical Center, Los Angeles, California 90073.
Am J Trop Med Hyg. 1988 Mar;38(2):342-7. doi: 10.4269/ajtmh.1988.38.342.
The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (male and female Schistosoma mansoni) with [3H- and 14C]-2-deoxy-D-glucose. The relative phosphorylation rate of 2-[3H]-2DG to 1-[14C]-D-glucose (i.e. the phosphorylation coefficient) was also measured in male and female schistosomes. Even though 2DG is taken up more rapidly than glucose, it is phosphorylated at a much slower rate in S. mansoni. Mated schistosomes phosphorylate 2DG and glucose at a greater rate than do unmated worms. In contrast, the phosphorylation coefficient is greater in separated than mated schistosomes. In schistosomes exposed to oltipraz for short time periods (6 min, at a concentration of 10 micrograms/ml) glucose utilization rates were significantly reduced in (both mated and separated) female S. mansoni and by a similar magnitude (not significant) in males.
通过用[³H-和¹⁴C]-2-脱氧-D-葡萄糖对血吸虫（曼氏血吸虫雌雄虫）进行连续脉冲来测定2-脱氧葡萄糖（2DG）的磷酸化速率。还测定了曼氏血吸虫雌雄虫中2-[³H]-2DG与1-[¹⁴C]-D-葡萄糖的相对磷酸化速率（即磷酸化系数）。尽管2DG的摄取比葡萄糖更快，但在曼氏血吸虫中其磷酸化速率要慢得多。已交配的血吸虫对2DG和葡萄糖的磷酸化速率比未交配的虫体更高。相比之下，分离的血吸虫的磷酸化系数比已交配的血吸虫更大。在短时间（6分钟，浓度为10微克/毫升）暴露于奥替普拉的血吸虫中，（已交配和分离的）曼氏血吸虫雌虫的葡萄糖利用率显著降低，雄虫的葡萄糖利用率也有类似程度的降低（不显著）。
