Cornford E M, Crane P D
Comp Biochem Physiol B. 1985;82(1):25-8. doi: 10.1016/0305-0491(85)90122-1.
The rate of phosphorylation of 2-deoxy-D-glucose (2dGlc) was determined by incubating Schistosoma mansoni in vitro in [3H]2-deoxy-D-glucose; 60 sec after exposure to the [3H]dGlc, [14C]dGlc was added to the medium, and metabolic activity was arrested at 2 min by immersion of the tissue in ice-cold silicone oil. Column chromatographic separation of the neutral [3H]- and [14C]dGlc from the [3H]- and [14C]2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H]dGlc phosphorylated in 2 min, and the proportion of [14C]dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. In male schistosomes derived from mouse infections 4.4 +/- 0.8% of the dGlc was phosphorylated each minute, and 4.2 +/- 0.9% in the females. Lower rates of phosphorylation were measured in schistosomes taken from hamsters where males phosphorylated 2.4 +/- 1.1% of the dGlc each minute, and in females 2.7 +/- 1.0%. These studies suggest the high rate of hexose utilization by schistosomes compares to the conscious rat brain, where 11% of the dGlc is phosphorylated each minute.
通过在体外将曼氏血吸虫置于[³H]2-脱氧-D-葡萄糖中孵育来测定2-脱氧-D-葡萄糖（2dGlc）的磷酸化速率；在暴露于[³H]dGlc 60秒后，向培养基中加入[¹⁴C]dGlc，然后在2分钟时通过将组织浸入冰冷的硅油中来停止代谢活性。通过柱色谱法从[³H]-和[¹⁴C]2-脱氧-D-葡萄糖-6-磷酸中分离出中性的[³H]-和[¹⁴C]dGlc，从而可以估算出2分钟内磷酸化的[³H]dGlc的量以及1分钟内磷酸化的[¹⁴C]dGlc的比例；因此，从单个组织样本中确定了磷酸化速率。在源自小鼠感染的雄性血吸虫中，每分钟有4.4±0.8%的dGlc被磷酸化，雌性为4.2±0.9%。在从仓鼠体内获取的血吸虫中测得的磷酸化速率较低，其中雄性每分钟磷酸化2.4±1.1%的dGlc，雌性为2.7±1.0%。这些研究表明，与清醒大鼠大脑相比，血吸虫的己糖利用率很高，清醒大鼠大脑每分钟有11%的dGlc被磷酸化。
