Laboratorio de Neurobiología Molecular y Celular, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, La Fama, Alcaldía Tlalpan, CP 14269, Ciudad de México, Mexico.
Laboratorio de Biología Celular y Tisular, Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretaría de la Defensa Nacional, Cerrada de Palomas S/N, Lomas de San Isidro, Alcaldía Miguel Hidalgo, CP 11200, Ciudad de México, Mexico.
Brain Res. 2021 May 1;1758:147337. doi: 10.1016/j.brainres.2021.147337. Epub 2021 Feb 4.
Cobalt protoporphyrin (CoPP) is a potent heme oxygenase-1 inductor that produces temporary hypophagia and chronic weight loss. A complete description of this effect and the underlying mechanisms are unknown. In this work, we challenged the ability of CoPP to produce changes in rat behavior and cellular alterations in the Nucleus Accumbens that would explain those effects. We subcutaneously administered 25 µmol/kg CoPP in female rats and determined body weight, food intake, hyperactivity, and anxiety-like behavior, as well as the number of neurons and glial cells in the Nucleus Accumbens. CoPP significantly reduced food intake, water consumption, and body weight. Behavioral tests showed that anxiety-like behaviors and locomotor activity were not modified five days after the administration of CoPP. We also found a reduced number of neurons in the Nucleus Accumbens Shell. The above results could be relevant to diseases like anorexia, so it is necessary to deepen the study about the molecular mechanisms involved in reducing the food intake and weight loss elicited by CoPP.
钴原卟啉（CoPP）是一种有效的血红素加氧酶-1诱导剂，可导致暂时的食欲减退和慢性体重减轻。目前尚不清楚这种作用及其潜在机制的完整描述。在这项工作中，我们挑战了 CoPP 产生大鼠行为变化和伏隔核细胞改变的能力，这些改变可以解释这些作用。我们给雌性大鼠皮下注射 25µmol/kg 的 CoPP，并测定体重、食物摄入量、活动度和焦虑样行为，以及伏隔核的神经元和神经胶质细胞数量。CoPP 显著减少了食物摄入量、水摄入量和体重。行为测试表明，CoPP 给药五天后，焦虑样行为和运动活性没有改变。我们还发现伏隔核壳中的神经元数量减少。上述结果可能与厌食症等疾病有关，因此有必要深入研究 CoPP 引起的食物摄入减少和体重减轻所涉及的分子机制。
